Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Induction and growth of adventitious roots and bioreactor culture in Codonopsis lanceolata

더덕 (Codonopsis lanceolata)의 부정근 유도 및 생장에 미치는 배양조건과 생물반응기 배양

  • Ahn, Chang-Ho (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Bae, Kee-Hwa (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Yi, Jae-Seon (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Choi, Yong-Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
  • 안창호 (강원대학교 산림환경과학대학 임학과) ;
  • 배기화 (강원대학교 산림환경과학대학 임학과) ;
  • 이재선 (강원대학교 산림환경과학대학 임학과) ;
  • 최용의 (강원대학교 산림환경과학대학 임학과)
  • Published : 2008.06.30
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Abstract

This paper reported the establishment of mass production system of adventitious roots of Codonopsis lanceolata through shake flask and bioreactor culture. Induction of adventitious roots was started from the explants of leaf, stem and root on 1/2 strength Murashing and Skoog (MS) solid medium. Stem segments were the best explants for induction of adventitious roots compared to leaf and root segments. Among the different auxins tested (NAA, IBA and IAA), number of adventitious root per explant was highest on solid medium with 1.0 mg/L IBA, and produced $9.9{\pm}1.2$ roots per explant. However, growth of adventitious roots was fast in the presence of IBA at low concentration (0.1 mg/L). In shake flask culture, maximum production of adventitious roots (fresh weight) was obtained in half-strength MS medium compared to full-strength and one-third MS medium. When the adventitious roots produced in shake-flask culture were transferred to 5 L air-lift bioreactor, 16 times of fresh weight increase was gained after one month of culture. These results indicate that this protocol for the production of C. lanceolata adventitious roots can be applied to large scale culture for practical application.

본 연구는 더덕의 부정근 배양조건과 생물반응기를 이용한 대량생산 체계를 확립하고자 수행하였다. 먼저 기내 식물체를 이용하여 잎, 줄기와 뿌리로부터 부정근을 유도하였다. 부정근의 유도는 줄기에서 가장 높은 결과를 보였다. 또한, 오옥신 종류별 (NAA, IBA와 IAA) 처리시 절편당 부정근 유도수는 1.0 mg/L IBA에서 $9.9{\pm}1.2$개로 가장 양호 하였다. 그렇지만 유도된 부정근의 길이생장은 0.1 mg/L IBA에서 가장 활발하였다. 최적조건의 액체배지 조성을 선발하고자 생체중 0.2 g의 부정근을 각각 MS, 1/2 MS 및 1/3 MS 액체배지가 든 250 mL 삼각플라스크에 접종한 결과 1/2 MS 배지에서 가장 양호한 생장을 보였다. 액체배지조건에서 진탕배양된 더덕 부정근으로 5 L 용량의 공기부양식 생물반응기에 4주간 배양한 결과 1/2 MS에 1.0 mg/L IBA가 첨가된 배지에서 대조구에 비해 16배의 증식이 이루어졌다. 본 연구에서는 더덕 식물체를 이용하여 부정근을 유도 및 증식에 필요한 배양 조건을 조사하였고, 유도된 부정근을 공기부양식 생물반응기에서 대량증식 할 수 있었다.

Keywords

References

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