Optimal Food and Concentration for the Growth of the Ultra-small Rotifer Synchaeta kitina

초소형 Rotifer Synchaeta kitina의 성장을 위한 최적 먹이 및 공급량

  • Park, Jin-Chul (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Park, Heum-Gi (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 박진철 (강릉대학교 해양생명공학부) ;
  • 박흠기 (강릉대학교 해양생명공학부)
  • Published : 2008.05.25

Abstract

We investigated the food-effect for ultra-small rotifer Synchaeta kitina cultured under a individual and community by several diets: 3 single trials(Tetraselmis suecica, TET; Isochrysis galbana, ISO; Marine Chlorella ellipsoidea, CHL) and 3 trials with a mixture of 2 species. The rotifer was cultured on the different feeding concentrations. In the individual cultures, the maximum number of offsprings and maximum lifespan of the female investigated to 5.8 inds. and 12.7 days in TET trial, respectively. Values of the developmental phases of the rotifer fed with T. suecica were higher than those of trials without T. suecica. Also it approached faster to maturation level. In the community cultures, the maximum density of TET+CHL trial elevated up to 1,569 inds./mL. But, CHL and ISO showed a poor growth rate and maximum density. The offsprings of the female increased continuously when fed by T. suecica trial, up to $10{\times}10^3$ cells/ind./day. As the quantity of supplied diet was lowered their lifespan were decreased. But, the maximum density and growth rate in the community cultures showed the highest value in the $10{\times}10^3$ cells/ind./day. The efficient food for mass culture of S. kitina was T. suecica, and optimum concentration of their food was 10,000 cells for an individual.

본 연구는 입이 작은 어류들의 초기 먹이생물로 이용 가능성이 있는 초소형 rotifer Synchaeta kitina의 대량배양을 위한 이들의 최적 먹이생물 및 공급량을 규명하는데 그 목적이 있다. 초소형 rotifer S. kitina를 대상으로 각기 다른 종류의 먹이를 6개(TET, ISO, CHL, TET+ISO, TET+CHL 및 ISO+CHL)의 실험구로 나누어 이들의 최고밀도, 포란률, 성장률, 발달단계, 산란수 및 수명 등을 조사하였다. 먹이종류에 따른 밀집배양에서 최고밀도, 포란률 및 성장률은 T. suecica를 단독 또는 혼합 공급한 실험구가 그렇지 않은 실험구에 비해 높은 것으로 나타났다. 또한 개체배양 실험에서도 생식 전 단계, 순 생식 단계, 산란수 및 수명이 T. suecica를 공급한 실험구에서 더 높은 것으로 조사되었다. 먹이 공급량에 따른 개체 및 밀집배양 실험에서는 rotifer 개체 당 T. suecica를 10,000세포로 공급하는 것이 최고밀도, 포란률, 성장률, 산란수 및 수명에 효율적인 것으로 나타났다. 따라서 초소형 rotifer S. kitina의 대량배양을 위한 최적의 먹이생물은 T. suecica이며, 그에 따른 공급량은 개체 당 10,000 세포가 적합할 것으로 판단된다.

Keywords

References

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