The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Effect of Injinho-tang in RAW 264.7 Cells

마우스 대식세포인 RAW 264.7 세포에서 인진호탕(茵陳蒿湯)의 항염증 효과

  • Yun, Hyun-Jeong (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Heo, Sook-Kyoung (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Yi, Hyo-Seung (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Chang-Hyun (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Byung-Wan (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center and Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University)
  • 윤현정 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실) ;
  • 허숙경 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실) ;
  • 이효승 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실) ;
  • 김창현 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실) ;
  • 김병완 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 심혈관계질환 천연물연구개발센터 방제학교실)
  • Published : 2008.06.30
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Abstract

Objectives : Inflammation is important event in the development of vascular diseases including hypertension, atherosclerosis, and restenosis. Injinho-tang(IJHT) has been used as a traditional Korean herbal medicine since ancient times, and today it is widely used as a medication for jaundice associated with inflammation of the liver. The aim of this study was to determine whether IJHT and its components inhibit production of nitrite, an index of NO, and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Methods : Cytotoxic activity of IJHT and its components on RAW 264.7 cells was using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were detected by western blot. Results : IJHT and its components significantly inhibited the LPS-induced NO production and iNOS expression accompanied by an attenuation of tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-6 (1L-6), IL-$1{\beta}$ and monocyte chemoattractant protein-1 (MCP-1) formation in macrophages. Conclusions : IJHT and its components inhibit LPS-induced inflammation via decreasing cytokines production. These results indicate that IJHT and its components have potential as an anti-inflammation and anti-artherosclerosis agent.

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