Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibiotic Resistances of Enterococcus Isolated from Salad and Sprout

샐러드와 새싹채소에서 Enterococcus의 분리와 항생제 내성특성

  • Kang, Tae-Mi (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Cho, Sun-Kyung (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Park, Jong-Hyun (Department of Food Science and Biotechnology, Kyungwon University)
  • 강태미 (경원대학교 식품생물공학과) ;
  • 조선경 (경원대학교 식품생물공학과) ;
  • 박종현 (경원대학교 식품생물공학과)
  • Published : 2008.06.28
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Abstract

To evaluate the antibiotic resistance of Enterococcus from salad and sprout, Enterococcus were isolated and identified from 47 salad samples and 37 sprout samples, and then their antibiotic resistances were analyzed. Ninety five Enterococcus, 41 strains from salad and 54 strains from sprout, were ultimately isolated. The frequent Enterococcus in salad and sprout were E. gallinarum, E. faecalis, E. faecium, E. hirae, and E. avium. Minimum inhibitory concentrations of the isolates for vancomycin were below $4{\mu}g/mL$, which were not high levels of resistance. All Enterococcus proved to be resistant to streptomycin and chloramphenicol. Twenty two percentage of the isolates were resistant to penicillin, however, almost the isolates were sensitive to tetracycline. Eighteen percentage of the isolates were resistant to erythromycin. All E. faecium and E. faecalis were found to be ampicillin-resistant, and seven E. faecalis and five E. faecium were resistant to rifampicin. Overall antibiotic resistances of Enterococcus isolates were relatively low and low resistance to vancomycin was similar to those evidenced by Enterococcus isolated from the other foods. Therefore, there may be no special risk from the antibiotics resistances of Enterococcus and especially vancomycin-resistant Enterococcus from the fresh-cut salads and the sprouts.

샐러드와 새싹채소에서 분리한 Enterococcus의 항생제 내성을 알아보기 위해서 샐러드 47개와 새싹채소 37개에서 Enterococcus를 분리하여 항생제 내성을 분석하였다. 총 95균주, 샐러드에서 41균주 새싹채소에서 54균주의 Enterococcus를 분리하였으며 대부분이 E. gallinarium, E. faecalis, E. faecium, E. hirae, E. casselifla편, E. avium으로 동정되었다. Vancomycin의 최소 생육 저해 농도는 $4{\mu}g/mL$ 이하로 샐러드와 새싹채소에서 분리된 Enterococcus에서는 vancomycin내성이 높지 않은 것으로 나타났다. 그리고 모든 Enterococcus에서 streptomycin과 chloramphenicol에 내성을 보였으며, 22% Enterococcus는 penicillin에서 저농도 내성을 보였다. 그러나 거의 모든 Enterococcus에서 tetracycline에 민감성을 나타내었다. 검출된 모든 E. faecium과 E. faecalis에서도 ampicillin 저농고 내성을 보였으며, 7균주의 E. faecium과 5균주의 E. faecalis에서 rifampin 저농도 내성을 보였다. 전반적으로 분리된 Enterococcus는 vancomvcin과 다른 항생제에 대해 감수성과 낮은 내성을 각각 보였으며 다른 식품 분리균주에서도 유사한 결과를 보이고 있다. 그러므로 샐러드와 새싹채소에서의 분리된 Enteroccocus는 vancomycin과 다른 항생제에도 내성이 낮아 특별히 항생제 위해정도는 크지 않은 것으로 보인다.

Keywords

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