Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Kinetics of Cell Growth and Cyclosporin A Production by Tolypocladium inflatum when Scaling Up from Shake Flask to Bioreactor

  • El Enshasy, H. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Fattah, Y. Abdel (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Atta, A. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Anwar, M. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Omar, H. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Magd, S. Abou El (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Zahra, R. Abou (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications)
  • Published : 2008.01.31
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Abstract

The kinetics of cell growth and Cyclosporin A (Cyc A) production by Tolypocladium inflatum were studied in shake flasks and bioreactors under controlled and uncontrolled pH conditions. In the case of the shake flask, the production time was extended to 226 h and the maximal antibiotic concentration was 76 mg/l. When scaling up the cultivation process to a bioreactor level, the production time was reduced to only 70h with a significant increase in both the cell growth and the antibiotic production. The maximal dry cell weights in the case of the controlled pH and uncontrolled pH cultures in the bioreactor were 22.4g/l and 14.2g/l, respectively. The corresponding maximal dry cell weight values did not exceed 7.25g/l with the shake flask cultures. The maximal values for Cyc A production were 144.72 and 131.4 mg/l for the controlled and uncontrolled pH cultures, respectively. It is also worth noting that a significant reduction was observed in both the dry cell mass and the antibiotic concentration after the Cyc A production phase, whereas the highest rate of antibiotic degradation was observed in the stirred tank bioreactor with an uncontrolled pH. Morphological characterization of the micromorphological cell growth (mycelial/pellet forms) was also performed during cultivation in the bioreactor.

Keywords

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