Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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$\beta$-Glucan Suppresses LPS-stimulated NO Production Through the Down-regulation of iNOS Expression and $NF{\kappa}B$ Transactivation in RAW 264.7 Macrophages

  • Yang, Jeong-Lye (Busan Newport Import Food Inspection Office, Busan Regional Food & Drug Administration) ;
  • Jang, Ji-Hyun (BK21 Center for Smart Food and Drugs, Biohealth Products Research Center, Inje University) ;
  • Radhakrishnan, Vinodhkumar (BK21 Center for Smart Food and Drugs, Biohealth Products Research Center, Inje University) ;
  • Kim, Yang-Ha (Department of Food and Nutrition, Ehwa U0mans University) ;
  • Song, Young-Sun (BK21 Center for Smart Food and Drugs, Biohealth Products Research Center, Inje University)
  • Published : 2008.02.29
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Abstract

The antioxidant and anti-inflammatory protective effects of $\beta$-glucan from barley on RAW 264.7 murine macrophage cells induced by lipopolysaccharide (LPS) were examined. The RAW 264.7 murine macrophages were preincubated with various concentrations ($0-200\;{\mu}g/mL$) of $\beta$-glucan and stimulated with LPS to induce oxidative stress and inflammation. The $\beta$-glucan treatments were found to reduce thiobarbituric acid-reactive substance (TBARS) accumulation, and enhance glutathione levels and the activities of antioxidative enzymes, including superoxide dismutase (SOD), catalase, glutathione reductase, and glutathione peroxidase (GSH-px) in the LPS-stimulated macrophages as compared to the LPS-only treated cells. Nitric oxide (NO) production was significantly suppressed in a dose-dependent manner (p<0.05) with an $IC_{50}$ of $104\;{\mu}g/mL$. Further treatment with $\beta$-glucan at $200\;{\mu}g/mL$ suppressed NO production to 2% of the LPS-control, and suppressed the levels of inducible nitric oxide synthase (iNOS) protein and mRNA in a dose-dependent manner. The specific DNA binding activity of nuclear factor ${\kappa}B\;(NF{\kappa}B)$ was significantly suppressed by $\beta$-glucan treatment with an $IC_{50}$ of $220\;{\mu}g/mL$ in a dose-dependent manner. Finally, barley $\beta$-glucan ameliorates NO production and iNOS expression through the down-regulation of $NF{\kappa}B$ activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages.

Keywords

References

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