Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Sasa quelpaertensis Leaf Extracts Induce Apoptosis in Human Leukemia HL-60 Cells

  • Jang, Mi-Gyeong (Technology Innovation Center for Life Science, Cheju National University) ;
  • Park, Soo-Young (Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Sun-Ryung (Department of Life Science, Cheju National University) ;
  • Choi, Soo-Youn (Technology Innovation Center for Life Science, Cheju National University) ;
  • Hwang, Joon-Ho (Technology Innovation Center for Life Science, Cheju National University) ;
  • Ko, Hee-Chul (Technology Innovation Center for Life Science, Cheju National University) ;
  • Park, Ji-Gweon (Technology Innovation Center for Life Science, Cheju National University) ;
  • Chung, Wan-Seok (Technology Innovation Center for Life Science, Cheju National University) ;
  • Kim, Se-Jae (Department of Life Science, Cheju National University)
  • Published : 2008.02.29
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Abstract

In east Asia, the leaves of various Sasa species have been used in folk medicine for centuries. The effects of the methanolic extract and its subsequent fractions derived from the leaves of Sasa quelpaertensis Nakai on cell proliferation in human leukemia HL-60 cells were evaluated. The ethyl acetate fraction of this extract (ESQL) significantly reduced cell viability in a dose-dependent manner ($0-250\;{\mu}g/mL$). ESQL ($IC_{50}=24.8\;{\mu}g/mL$) exhibited growth inhibition comparable to the main constituent of green tea, epigallocatechin ($IC_{50}=26.2\;{\mu}g/mL$), which was used as a positive control. ESQL treatment induced apoptosis, which was confirmed by the presence of nuclear condensation and annexin V-staining. These results demonstrate that ESQL contains chemopreventive phytochemicals that may be useful in neutraceutical applications.

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References

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