Antimicrobial Activities of White, Red, and Extruded Ginsengs with Different Extraction Conditions

  • Norajit, Krittika (Department of Food Science and Technology, Kongju National University) ;
  • Park, Mi-Ja (Department of Food Nutrition, Kongju National University) ;
  • Ryu, Gi-Hyung (Department of Food Science and Technology, Kongju National University)
  • Published : 2008.08.31

Abstract

White, red, and extruded ginsengs were studied against 8 strains of food-borne pathogens and/or food spoilage microorganisms. The ginseng powders were extracted with different extractants and screened for antimicrobial activity using the disc diffusion and broth dilution techniques. The results showed that the yield of extraction was higher with increase of aqueous solution content and temperature. Preliminary screening revealed that the red ginseng extracts were most active, that has been found to be highly effective against all tested microbe except Listeria monocytogenes. Moreover, Bacillus subtilis has shown highly susceptible, which the diameters of inhibition zone values of 28 extracts were between 7 and 14 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) recorded for the different crude ginseng extracts against microorganism using ranged from 6.25 to 100 mg/mL, indicated that the methanol extract of ginseng were more effective than ethanol and water extracts. The 60% methanol extract of red ginseng had the greatest effects against B. subtilis with MIC and MBC values at 6.25 mg/mL.

Keywords

References

  1. Mead PS, Slutsker L, Dietz V, McCaig LF, Breese JS, Shapiro C, Griffin PM, Tauxe RV. Food related illness and dead in the United States. Emerg. Infect. Dis. 5: 607-625 (1999) https://doi.org/10.3201/eid0505.990502
  2. Garcia MT, Canamero MM, Lucas R, Omar NB, Pulido RP, Galvez A. Inhibition of Listeria monocytogenes by enterocin EJ97 produced by Enterococcus faecalis EJ97. Int. J. Food Microbiol. 90: 161-170 (2004) https://doi.org/10.1016/S0168-1605(03)00051-5
  3. Nychas GJE. Natural antimicrobials from plants. pp. 53-89. In: New Methods of Food Preservation. Gould GW (ed). Blackie Academic & Professional, Glasgow, UK (1995)
  4. Tang W, Eisenbrand G. Panax ginseng C.A. Meyer. pp. 711-737. In: Chinese Drugs of Plant Origin. Tang W, Eisenbrand S (eds). Springer, Berlin, Germany (1992)
  5. Park JD. Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C. A. Meyer). Korean J. Ginseng Sci. 20: 389-415 (1996)
  6. Kwon JH, Belanger JMR, Pare JRJ, Yaylayan V. Application of the microwave-assisted process (MAP) to the fast extraction of ginseng saponins. Food Res. Int. 36: 491-498 (2003) https://doi.org/10.1016/S0963-9969(02)00197-7
  7. Court WA, Hendel JG, Elmi J. Reversed-phase high performance liquid chromatographic determination of ginsenosides of Panax quinquefolium. J. Chromatogr. A 755: 11-17 (1996) https://doi.org/10.1016/S0021-9673(96)00580-8
  8. Ji QC, Harkey MR, Henderson GL, Gershwin ME, Stern JS, Hackman RM. Quantitative determination of ginsenosides by high performance liquid chromatography tandem mass spectrometry. Phytochem. Analsis 12: 320-326 (2001) https://doi.org/10.1002/pca.593
  9. Zhu S, Zou K, Cai S, Meselhy MR, Komatsu K. Simultaneous determination of triterpene saponins in ginseng drugs by high performance liquid chromatography. Chem. Pharm. Bull. 52: 995-998 (2004) https://doi.org/10.1248/cpb.52.995
  10. Yokozawa T, Oura H. Facilitation of protein biosynthesis by ginsenoside-Rb2 administration in diabetic rats. J. Nat. Prod. 53: 1514-1518 (1990) https://doi.org/10.1021/np50072a018
  11. Yokozawa T, Fujitsuka N, Yasui T, Oura H. Effects of ginsenoside-Rb2 on adenine nucleotide content of rat hepatic tissue. J. Pharm. Pharmacol. 43: 290-291 (1991) https://doi.org/10.1111/j.2042-7158.1991.tb06689.x
  12. Estevez-Braun A, Estevez-Reyes R, Moujir LM, Ravelo AG, Gonzalez AG. Antibiotic activity and absolute configuration of 8Sheptadeca-2(Z), 9(Z)-diene-4,6-diyne-1,8-diol from Bupleurum salicifolium. J. Nat. Prod. 57: 1178-1182 (1994) https://doi.org/10.1021/np50110a009
  13. Lam SK, Ng TB. Pananotin, a potent antifungal protein from roots of the traditional Chinese medicinal herb Panex ginseng. Planta Med. 68: 1024-1028 (2002) https://doi.org/10.1055/s-2002-35649
  14. NCCLS. Document M2-A7. Performance standards for antimicrobial disk susceptibility tests. National Committee for Clinical Laboratory Standards, Wayne, PA, USA (2001)
  15. Vanden Berghe DA, Vlietinck AJ. Screening methods for antibacterial and antiviral agents from higher plants. Vol. 6, pp. 47-69. In: Methods in Plant Biochemistry Assays for Bioactivity. Dey PM, Harborne JB, Hostettman K (eds). Academic Press, San Diego, CA, USA (1991)
  16. Moure A, Cruz JM, Franco D, Dominguez JM, Sineiro J, Dominguez H. Natural antioxidants from residual sources-a review. Food Chem. 72: 145-171 (2001) https://doi.org/10.1016/S0308-8146(00)00223-5
  17. Hao YY, Brackett RE, Doyle MP. Inhibition of Listeria monocytogenes and Aeromonas hydrophila by plant extracts in refrigerated cooked beef. J. Food Protect. 61: 307-312 (1998) https://doi.org/10.4315/0362-028X-61.3.307
  18. Benny KHT, Vanitha J. Immunomodulatory and antimicrobial effects of some traditional Chinese medicinal herbs: A review. Curr. Med. Chem. 11: 1423-1430 (2004) https://doi.org/10.2174/0929867043365161
  19. Kwak YS, Hwang MS, Kim SC, Kim CS, Do JH, Park CK. A growth inhibition effect of saponin from red ginseng on some pathogenic microorganisms. J. Ginseng Res. 30: 128-131 (2006) https://doi.org/10.5142/JGR.2006.30.3.128
  20. Zhang S, Chen R, Wu H, Wang C. Ginsenoside extraction from Panax quinquefolium L. (American ginseng) root by using ultrahigh pressure. J. Pharmaceut. Biomed. Anal. 41: 57-63 (2006) https://doi.org/10.1016/j.jpba.2005.10.043
  21. Galbraith H, Miller TB. Effect of long-chain fatty acids on bacterial respiration and amino acid uptake. J. Appl. Bacteriol. 36: 659-675 (1973) https://doi.org/10.1111/j.1365-2672.1973.tb04151.x
  22. Skocibusic M, Bezic N, Dunkic V. Phytochemical composition and antimicrobial activities of the essential oils from Satureja subspicata Vis. growing in Croatia. Food Chem. 96: 20-28 (2006) https://doi.org/10.1016/j.foodchem.2005.01.051