Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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THE HYPERMETHYLATION OF E-CADHERIN GENE IN ORAL SQUAMOUS CELL CARCINOMA

구강 편평세포암에서 E-cadherin 유전자의 과메틸화

  • Pyo, Sung-Woon (Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea) ;
  • Kim, Young-Sill (Department of Pathology, College of Medicine, Cheju National University) ;
  • Park, Ji-Young (Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea) ;
  • Kim, Chang-Hyen (Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea) ;
  • Lee, Won (Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea) ;
  • Park, Min-Kyu (Department of Oral and Maxillofacial Surgery, College of Medicine, The Catholic University of Korea)
  • 표성운 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 김영실 (제주대학교 의과대학 병리학교실) ;
  • 박지영 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 김창현 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 이원 (가톨릭대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박민규 (가톨릭대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2008.04.30
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Abstract

Loss of E-cadherin (E-cad) expression has been found in multiple cancers and is postulated to facilitate tumor cell dissociation and metastais. Promotor methylation may provides an alternative pathway for loss of gene function. This study evaluated the role of hypermethylation in the down-regulation of E-cad in oral squamous cell carcinoma (OSCC). We examined the E-cad expression by immunohistochemical staining and detected methylation status by methylation-specific polymerase chain reaction (MSP) in 20 OSCC tissues. Overally, 12 (60%) cases of hypermethylation of E-cad were detected and we found there were no correlation between methylation and age, histologic grade, lympn node metastasis, tumor size and clinical stage. However, Eleven (73.3%) of 15 samples which was negative for E-cad staining showed hypermethylation of E-cad promotor region. On the other hand, only one (20%) of 5 E-cad positive sample was observed with methylated status. The underexpression of E-cad was found to be related to promotor hypermethylation (p=0.035). In conclusion, we suggest that hypermethylation play a role in inactivation of E-cad gene and may be a appreciable biomarker for diagnosis and treatment of OSCC.

Keywords

References

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