Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Genetic Diversity of Endangered Fish Hemibarbus mylodon (Cyprinidae) Assessed by AFLP

AFLP 분석에 의한 어름치 Hemibarbus mylodon의 유전 다양성

  • Lee, Yoon-A (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Yun, Young-Eun (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 이윤아 (순천향대학교 해양생명공학과) ;
  • 윤영은 (순천향대학교 해양생명공학과) ;
  • 남윤권 (부경대학교 양식학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Published : 2008.08.25
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Abstract

Korean spotted barbel Hemibarbus mylodon, is an endangered and endemic freshwater species in the Korean peninsula. Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity and population genetic structure of three populations (Bukhan, Namhan and Imjin-river). Fifteen AFLP primer pairs produced 795 products of which 135 were polymorphic(17%). Percentages of polymorphic bands were similar among the three populations, with accounting 11.9%, 11.1%, and 13.4% for Bukhan, Namhan and Imjin-river populations, respectively. An average genetic similarity among the three populations was 0.969. The average heterozygosity (0.033-0.040) and genetic diversity (0.036-0.043) were significantly low. Pairwise distance and fixation index analyses of three populations also suggested quite a low genetic differentiation one another. These results would provide a fundamental baseline data to develop the effective strategy for the management and restoration of this endangered fish species.

한국 고유종이며 멸종위기에 처해 있는 어름치 Hemibarbus mylodon 3집단 (북한강, 남한강, 임진강)의 유전적 다양성 및 집단의 유전적 구조를 분석하기 위하여 AFLP분석을 실시하였다. 총 15 primer 쌍을 이용한 3집단의 AFLP 분석에서 795개의 bands가 생성되었으며, 집단 내 다형 band의 출현 빈도는 3개 집단에서 북한강 11.9%, 남한강 11.1%, 임진강 13.4%로 유사하게 나타났고, 평균 유사도는 96.6%로 나타났다. 평균 이형 접합율(0.033-0.040)과 유전적 다양도(0.036-0.043)는 매우 낮은 값을 보였다. 3개의 집단의 Pairwise distance 및 pairwise fixation index 역시 매우 낮은 값을 나타내어 본 연구에서 분석한 어름치 3개 집단은 유전적으로 매우 밀접한 근연관계를 나타내었다. 이러한 결과는 추후 본 종의 복원 및 관리에 필요한 기초자료로 활용될 수 있을 것이다.

Keywords

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