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Physiological Functionality and Enzyme Activity of Biomass from Pichia anomala Grown on Ginseng-Steaming Effluent

  • Kim, Na-Mi (KT&G Co. Central Research Institute) ;
  • So, Seung-Ho (KT&G Co. Central Research Institute) ;
  • Lee, Sung-Gae (KT&G Co. Central Research Institute) ;
  • Song, Jung-Eun (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Seo, Dong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
  • Published : 2008.09.30

Abstract

A novel biomass was prepared from Pichia anomala KCCM 11473, which grew well in ginseng-steaming effluent (GSE), and its physiological functionalities and enzyme activities were determined. When the strain was cultured in the GSE (pH 6.0) at 30$^{\circ}C$ for 48 h, 1.6 mg of biomass per ml-cultures was produced. The cell-free extract of the biomass showed high antihypertensive angiotensin I-converting enzyme inhibitory activity of 72.0% and anticholesteromia HMG-CoA reductase inhibitory activity of 46.5%. The cell-free extract also showed 13.0 U per ml and 8.5 U per ml of neutral protease activity and alkaline protease, respectively.

Keywords

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