Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Induction of Apoptosis by Scolopendra subspinipes mutilans in Human Leukemia HL-60 Cells through Bcl-xL Regulation

왕지네(Scolopendra subspinipes mutilans)의 Bcl-xL 조절에 의한 HL-60 세포의 아폽토시스(Apoptosis) 유도

  • 김길남 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 김상범 (오현중학교) ;
  • 윤원종 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 양경식 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 박수영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소)
  • Published : 2008.11.28
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Abstract

The anticancer properties of Scolopendra subspinipes mutilans extract were investigated. The extract from S. subspinipes mutilans by 80% EtOH was fractionated with n-hexane, dichloromethan ($CH_2Cl_2$), ethylacetate (EtOAc), and butanol (BuOH) in order. The EtOAc fraction showed the highest inhibitory activity (about 80%) against human leukemia (HL-60) cell growth at $50\;{\mu}g/mL$. To explore the mechanism of cytotoxicity, we used several measures of apoptosis to determine whether these processes were involved in EtOAc fraction-induced HL-60 cell death. Our results showed EtOAc fraction induced cell shrinkage, cell membrane blebbing, apoptotic body, and DNA fragmentation. The EtOAc fraction gradually decreased the expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3, -9 and cleavage of PARP. These findings suggest that S. subspinipes mutilans exhibits potential anticancer properties.

왕지네 에탄올 추출물 및 순차적 분획물 중 디클로로메탄과 에틸아세테이트 분획물에서 HL-60 세포 성장이 강하게 억제되었다. 또한, 디클로로메탄과 에틸아세테이트 분획물을 처리하였을 때 apoptosis의 특징인 DNA 절편화, 핵의 응축과 apoptotic body가 관찰되었다. 이는 왕지네 분획물의 암세포성장 억제가 apoptosis에 의해 억제되는 것을 알 수 있었다. 디클로로메탄과 에틸아세테이트 분획물의 apoptosis 유도는 anti-apoptosis 단백질인 Bcl-xL의 억제를 통하여 apoptosis 유도가 시작되고 세포사멸에 직접적으로 영향을 미치는 casapse-3과 PARP의 활성을 일으켜 apoptosis를 유도하였다. 본 연구는 왕지네의 항암효과를 과학적 근거를 제시하고 기능성식품이나 항암제로 개발할 있는 가능성을 제시하였다. 향후 왕지네를 이용한 제품의 개발을 위해서는 유효성분의 동정 및 그 성분의 작용 기전에 대한 추가 연구가 필요할 것으로 보인다.

Keywords

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