Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Proteomic analysis of Korean ginseng(Panax ginseng C. A. Meyer) following exposure to salt stress

  • Kim, Sun-Tae (Environmental Biotechnology National Core Research Center and PMBBRC, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Laboratory in Gyeongsang National University) ;
  • Lee, Kyung-Hee (Division of Applied Life Science(BK21 Program), Graduate School of Gyeongsang National University) ;
  • Hwang, Jung-Eun (Division of Applied Life Science(BK21 Program), Graduate School of Gyeongsang National University) ;
  • Bang, Kyong-Hwan (Ginseng & Medicinal Plants Research Institute, NICS, RDA) ;
  • Kim, Young-Chang (Ginseng & Medicinal Plants Research Institute, NICS, RDA) ;
  • Kim, Ok-Tae (Ginseng & Medicinal Plants Research Institute, NICS, RDA) ;
  • Yoo, Nam-Hee (Research Center for Industrial Development of BioFood Materials, Chonbuk National University) ;
  • Kang, Kyu-Young (Division of Applied Life Science(BK21 Program), Graduate School of Gyeongsang National University) ;
  • Hyun, Dong-Yun (Ginseng & Medicinal Plants Research Institute, NICS, RDA) ;
  • Lim, Chae-Oh (Environmental Biotechnology National Core Research Center and PMBBRC, Gyeongsang National University)
  • Published : 2008.09.30
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Abstract

We evaluated the response to salt stress of two different ginseng lines, STG3134 and STG3159, which are sensitive and tolerant, respectively, to salt treatment. Plants were exposed to a 5 dS/m salt solution, and chlorophyll fluorescence was measured. STG3134 ginseng was more sensitive than STG3159 to salt stress. To characterize the cellular response to salt stress in the two different lines, changes in protein expression were investigated using a proteomic approach. Total protein was extracted from detached salt-treated leaves of STG3134 and STG3159 ginseng, and then separated by two-dimensional polyacrylamide gel electrophoresis(2-DE). Approximately 468 protein spots were detected by 2-DE and Coommassie brilliant blue staining. Twenty-two proteins were found to be reproducibly up- or down-regulated in response to salt stress. Among these proteins, twelve were identified using MALDI-TOF MS and ESI-Q-TOF and classified into several functional groups: photosynthesis-related proteins(oxygen-evolving enhancer proteins 1 and 2, rubisco and rubisco activase), detoxification proteins(polyphenol oxidase) and defense proteins($\beta$-1,3-glucanase, ribonuclease-like storage protein, and isoflavone reductase-like protein). The protein levels of ribonuclease-like storage protein, which was highly induced in STG3159 ginseng as compared to STG3134, correlated tightly with mRNA transcript levels, as assessed by reverse-transcription(RT)-PCR. Our results indicate that salinity induces changes in the expression levels of specific proteins in the leaves of ginseng plants. These changes may, in turn, playa role in plant adaptation to saline conditions.

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References

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