Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Protective Effect of Green Tea Extract on Amyloid $\beta$ peptide-induced Neurotoxicity

아밀로이드베타 펩타이드 유도성 신경세포독성에 대한 녹차 추출물의 보호 효과

  • Kim, Young-In (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Park, Jeong-Yoon (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Choi, Soo-Jung (Graduate School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Jae-Kyeom (Department of Food and Biotechnology, Korea University) ;
  • Jeong, Chang-Ho (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Lee, Seung-Cheol (Division of Food and Biotechnology, Kyungnam University) ;
  • Cho, Sung-Hwan (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Heo, Ho-Jin (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 김영인 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 박정윤 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 최수정 (고려대학교 생명공학원) ;
  • 김재겸 (고려대학교 식품생명공학과) ;
  • 정창호 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 조성환 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부.농업생명과학연구원)
  • Published : 2008.10.31
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Abstract

Amyloid $\beta$ peptide ($A{\beta}$) is known to increase oxidative stress in nerve cells, leading to apoptosis that is characterized by free radical formation and lipid peroxidation. Neurodegenerative diseases such as Alzheimer's disease (AD) are characterized by large deposits of $A{\beta}$ in the brain. In our study, neuronal protective effects of green tea, along with water activity (0.813), and leaf storage periods (fresh leaf, or leaf stored for up to 4 weeks) were investigated. We measured protective effects against $A{\beta}$-induced cytotoxicity in neuron-like PC12 cells. Powdered green tea was extracted with distilled water at $70^{\circ}C$ for 5 min, and this extract was freeze-dried and stored at $-20^{\circ}C$ until use. In cell viability assays using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), the fresh extract, and that obtained after 1 week of leaf storage, showed the best protective effects against $A{\beta}$-induced neurotoxicity. As oxidative stress causes membrane breakdown, the protective effect of green tea extracts was investigated using lactate dehydrogenase (LDH) and trypan blue exclusion assays. LDH release into the medium was inhibited (by 20-25%) in all tests. In addition, all green tea extracts (fresh, or stored before extraction for up to 4 weeks) showed better cell protective effects ($93.3{\pm}1.8-96.2{\pm}2.4$) than did vitamin C ($91.0{\pm}1.6$), used as a positive control. The results suggest that effectiveness of green tea extracts falls with prolonged leaf storage.

Amyloid $\beta$ peptide($A{\beta}$)은 지방산화 및 free radical의 생산에 의해 신경세포의 apoptosis를 유도하거나 산화적 스트레스를 증가시키는 원인이 되는 물질로 알려져 있으며, 알츠하이머와 같은 신경계 질환은 뇌에 아밀로이드베타 단백질들의 축적에 의해서 일어난다. 따라서 본 연구에서는 수분 활성도 0.813인 분말 녹차를 저장기간별로 저장한 후 $70^{\circ}C$에서 5분간 추출한 분말 녹차 열수추출물을 이용하여 아밀로이드 베타단백질에 의해 유도된 신경세포 보호효과를 측정하였다. 아밀로이드 베타 단백질에 의해 유도된 PC 12 신경세포에 대한 보호효과를 MTT방법으로 측정한 결과 저장하지 않은 시료와 1주일 동안 저장한 분말 녹차 열수추 출물에서 가장 높은 보호효과를 보였다. 저장기간에 따른 분말 녹차 열수추출물의 산화적 스트레스에 의한 세포막 보호효과를 LDH 및 trypan blue exclusion 방법으로 측정한 결과 모든 시료에서 $20{\sim}25%$의 LDH release 보호효과를 보였으며, 또한 trypan blue exclusion 실험에서 positive control로 사용한 $91.0{\pm}1.6%$의 vitamin C 보다 분말 녹차 열수추출물에서 $96.3{\pm}1.8{\sim}96.2{\pm}2.4%$로 더 높은 세포막 보호효과를 보였다.

Keywords

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