Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of Aqueous Chlorine Dioxide and Citric Acid on Reduction of Salmonella typhimurium on Sprouting Radish Seeds

이산화염소수 및 구연산처리에 따른 무(Raphanus sativus L.) 새싹과 종자의 미생물 제어 효과

  • Published : 2008.10.31
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Abstract

The effect of citric acid-aqueous chlorine dioxide ($ClO_2$) treatment of radish seeds artificially contaminated with Salmonella typhimurium was studied. Radish seeds were inoculated by immersion, in more than 106 log CFU/g seed, of a suspension of S. typhimurium, dried, and stored sealed at $4^{\circ}C$ Radish seeds soaked in 200 ppm aqueous ClO2 solution for 10 min showed a bacterial reduction of 1.08 log CFU/g seed, and the lowering of microbial burden noted in seeds soaked in 2% (w/v) citric acid solution for 10 min was 2.89 log CFU/g seed. Next, radish seeds were exposed to 0.5% (v/v) glycerol solution for 10 min either before or after treatment with 200 ppm aqueous ClO2 or 2% (w/v) citric acid for 10 min. Glycerol exposure after citric acid treatment reduced bacteria by 3.46 log CFU/g seed, and glycerol treatment after aqueous $ClO_2$ treatment reduced the microbial burden by 2.14 log CFU/g seed. Both glycerol treatments yielded better elimination of S. typhimurium than did a single treatment with either citric acid or aqueous $ClO_2$. Radish seeds inoculated with S. typhimurium were treated throughout the entire growth period. Although radish seed treatment was effective, treatment by citric acid and aqueous $ClO_2$ after sprouting was not effective to eliminate S. typhimurium.

새싹 채소 중 무순의 위생적인 재배 조건을 설정하기 위하여 종자세척 및 관수로서 이산화염소수, citric acid 및 glycerin의 적용 효과를 조사하였다. 무 종자에 인위적으로 오염시키기 위하여 Salmonella typhimurium은 실험실 조건에서 배양하였으며, 무 종자를 S. typhimurium 현탁액에 침지시켜 균수를 약 $10^6\;log\;CFU/g$로 조절하였다. 무 종자에 대한 세척 효과는 이산화염소수 및 citric acid의 단독처리보다 병행처리시 더욱 우수한 효과를 나타내어, 이산화염 소수 200 ppm과 citric acid 2%의 병행처리시 2.89 log CFU/g 의 감소효과를 나타내었다. 또한, glycerol 0.5%/citric acid 2.0% 처리의 경우 3.46 log CFU/g, glycerol 0.5%/이산화염 소수 200 ppm 처리는 2.14 log CFU/g의 S. typhimurium 균의 감소를 나타내어, glycerol 단독 처리보다 병행처리구가 더 우수한 효과를 나타내었다. 무 종자의 발아 및 성정에 따른 균수 감소는 비록 종자에 대한 처리효과가 유의적으로 있었음에도 불구하고 새싹 성장 후 균수의 차이는 미미하였다.

Keywords

References

  1. Feng, P. (1997) A summary of background information and foodborne illness associated with the consumption of sprouts. Center for Food Safety and Applied Nutrition, Washington, DC
  2. Ponka, A., Anderson, Y., Siitonen, A., deJong, B., Jahkola, M., Haikapa, M., Kuhmooen, A. and Pakkala, Z.P. (1995) Salmonella in alfalfa sprouts. Lancet, 345, 462-463
  3. Ministry of Health and Welfare of Japan (1996) National Institute of Infectious Disease Control Division. Verocytotoxin-producing Escherichia coli (enterohemorrhagic E. coli) infection. Japan. Infect. Agents Surveill. Rep., 18, 153-154
  4. Fu, T., Stewart, D., Reineke, K., Ulaszek, J., Schlesser, J. and Tortorello, M. (2001) Use of spent irrigation water for microbiological analysis of alfalfa sprouts. J. Food Prot., 64, 802-806 https://doi.org/10.4315/0362-028X-64.6.802
  5. Gandhi, M., Golding, S., Yaron, S. and Matthews, K.R. (2001) Use of green fluorescent protein expressing Salmonella stanley to investigate survival, spatial location, and control on alfalfa sprouts. J. Food Prot., 64, 1891-1898 https://doi.org/10.4315/0362-028X-64.12.1891
  6. Andrews, W.H., Mislivec, P.B., Wilson, C.R., Bruce, V.R., Poelma, P.L., Gibson, R., Trucksess, M.W. and Young, K. (1982) Microbial hazards associated with bean sprouting. J. Assoc. Off. Anal. Chem., 65, 241-248
  7. Jaquette, C.B., Beuchat, L.R. and Mahon, B.E. (1996) Effecicacy of chlorine and heat treatment in killing Salmonella stanley inoculated onto alfalfa seeds and storage. Appl. Environ. Microbiol., 62, 2212-2215
  8. Bari, M.L., Nazuka, E., Sabina, Y., Todoriki, S. and Isshiki, K. (2003) Chemical and irradiation treatments for killing Escherichia coli O157:H7 on alfalfa, Radish, and mung bean seeds. J. Food Prot., 66, 767-774 https://doi.org/10.4315/0362-028X-66.5.767
  9. Splittstoesser, D.F., Queale, E.T. and Andaloro, B.W. (1983) The microbiology of vegetable sprouts during commercial production. J. Food Safety., 5, 79-86 https://doi.org/10.1111/j.1745-4565.1983.tb00458.x
  10. Fett, W.F. (2002) Reduction of Escherichia coli O157:H7 and Salmonella spp. on baboratory inoculated mung bean seed by chlorine treatment. J. Food Prot., 65, 848-852 https://doi.org/10.4315/0362-028X-65.5.848
  11. National Advisory Committee on Microbiological Criteria for Foods (NACMCF) (1999) Microbiological safety evaluations and recommendations on sprouted seeds. Int. J. Food Microbiol., 52, 123-153 https://doi.org/10.1016/S0168-1605(99)00135-X
  12. Delaquis, P.J., Sholberg, P.L. and Stanich, K. (1999) Disinfection of Mung bean seed with gaseous acetic acid. J. Food Prot., 62, 953-957 https://doi.org/10.4315/0362-028X-62.8.953
  13. Himathongkham, S., Nuanualsuwan, S., Riemann, H. and Cliver, D.O. (2001) Reduction of Escherichia coli O157:H7 and Salmonella typhimurium in artificially contaminated alfalfa seeds and mung beans by fumigation with ammonia. J. Food Prot., 64, 1817-1819 https://doi.org/10.4315/0362-028X-64.11.1817
  14. Kim, C., Hung, Y.C., Brackett, R.E. and Lin, C.S. (2003) Efficacy of electrolyzed oxidizing water in inactivating Salmonella on alfalfa seeds and sprouts. J. Food Prot., 66, 208-214 https://doi.org/10.4315/0362-028X-66.2.208
  15. Weiss, A. and Hammes, W.P. (2003) Thermal seed treatment to improve the food safety status of sprouts. J. Appl. Bot., 77, 152-155
  16. Kim, I.D. and Kim, S.D. (2001) Changes in quality of soybean sprouts grown by ozone water treatment during storage. Korean J. Food Preserv., 8, 279-384
  17. Kim, I.D., Park, M.J., Cho, J.W., Soe, S.S., Kim, M.K., Lee, J.B., Lee, S.K. and Kim, S.D. (1998) Effect of ozone treatment on the quality of soybean sprouts. Korean J. Food Preserv., 5, 177-185
  18. Kraybill, H.F. (1978) Origin, classification and distribution of chemicals in drinking water with an assessment of their carcinogenic potential. Vol. 1, pp. 211-228. In: Water chlorination. Jolly RL (ed). Ann Arbor Science, Ann Arbor, MI, USA
  19. Moore, G.S., Calabrese, E.J., DiNardi, S.R. and Tuthill, R.W. (1978) Potential health effect of chlorine dioxide as a disinfectant in potable water supplies. Med. Hypotheses, 4, 481-496 https://doi.org/10.1016/0306-9877(78)90017-8
  20. Kim, J.M. (2001) Use of chlorine dioxide as a biocide in the food industry. Food Ind. Nutr., 6. 33-39
  21. Ingram, M., Ottoway, F.J.H. and Coppock, J.B.M. (1956) The preservative action of acid substances in food. Chem. Ind., 42, 1154-1163
  22. Luck, E. and Jager, M. (1997) Antimicrobial food additives(2nd ed.). Springer, New York, USA, p.116-119
  23. Macris, B.J. (1975) Mechanisms of benzoic acid uptake by Saccharomyces cerevisiae. J. Appl. Microbiol., 30, 503-506
  24. Conner, D.E., Kotrola, J.S., Mikel, W.B. and Tamblyn, K.C. (1997) Effect of acetic-lactic acid treatment applied to beef trim on populations of Escherichia coli O157:H7 and Listeria monocytogenes in ground beef. J. Food Prot., 60, 1560-1563 https://doi.org/10.4315/0362-028X-60.12.1560
  25. Cutter, C.N., Dorsa, W.J. and Siragusa, G.R. (1997) Parameters affection the efficacy of spray washes against Escherichia coli O157:H7 and fecal contamination. J. Food Prot., 60, 614-618 https://doi.org/10.4315/0362-028X-60.6.614
  26. Prasai, R., Kastner, C., Kenney, P., Kropf, D., Fung, D. and Mease, L. (1997) Microbiological quality of beef subprimals as affected by lactic acid sprays applied at various points during vacuum storage. J. Food Prot., 60, 795-798 https://doi.org/10.4315/0362-028X-60.7.795
  27. APHA. (1995) Standard methods for the examination of water and wastewater. 19th ed. DPD Method. American 28. Public Health Association, Washington DC, USA, p. 4-76
  28. SAS User's Guide. Ver.6.12. (1995) Statistical Analysis Systems Institute, SAS Institute, Inc. Cary, NC, USA
  29. Abdul-Raouf, U.F., Beuchat, L.R., and Ammar, M.S. (1993) Survival and growth of Escheriachia coli O157:H7 in ground roasted beef as affected by pH, acidulants, and temperature. Appl. Environ. Microbiol., 59, 2364-2368
  30. Weissinger, W.R. and Beuchat, L.R. (2000) Comparison of aqueous chemical treatments to eliminate Salmonella on alfalfa seeds. J. Food Protect., 63, 1475-1782 https://doi.org/10.4315/0362-028X-63.11.1475
  31. Singh, N., Singh, R.K. and Bhunia, A.K. (2003) Sequential disinfection of Escherichia coli O157:H7 inoculated alfalfa seeds before and during sprouting using aqueous chlorine dioxide, ozonated water, and thyme essential oil. Lebensm.-Wiss. U.-Technol., 36, 235-243
  32. Taormina, P.J. and Beuchatn, L.R. (1999) Comparison of chemical treatment to eliminate enterohemorrhagic Escherichia coli O157:H7 on alfalfa seeds. J. Food Prot., 62, 318-324 https://doi.org/10.4315/0362-028X-62.4.318
  33. Lang, M.M., Ingham, B.H. and Ingham, S.C. (2000) Efficicacy of novel organic acid and hypochlorite treatments for eliminating Escherichia coli O157:H7 from alfalfa seeds prior to sprouting. Int. J. Food Microbiol., 58, 73-82 https://doi.org/10.1016/S0168-1605(00)00297-X
  34. Kroll, R.G. and Patchett, R.A. (1992) Induced acid tolerance in Listeria monocytogenes. Lett. Appl. Microbiol., 14, 224-227 https://doi.org/10.1111/j.1472-765X.1992.tb00691.x
  35. Hill, C., O'Driscoll, B. and Booth, I. (1995) Acid adaptation and food poisoning microorganisms. Int. J. Food Microbiol., 28, 245-254 https://doi.org/10.1016/0168-1605(95)00060-7
  36. O'Driscall, B., Gahan, G.M. and Hill, C. (1996) Adaptive acid tolerance response in Listeria monocytogenes: isolation of an acid-tolerant mutant which demonstrates increased virulence. Appl. Environ. Microbiol., 62, 1693-1698
  37. Goodson, M. and Rowbury, R.J. (1989) Resistance of acid-habituated Escherichia coli to organic acids and its medical and significance. Lett. Appl. Microbiol., 8, 211-214 https://doi.org/10.1111/j.1472-765X.1989.tb00250.x
  38. Cheng, H.Y. (1999) Survival of acid-adapted Escherichia coli O157:H7 in some acid foods and subsequent environmental stress. MS thesis, National Taiwan University, Taipei, Taiwan.
  39. Foster, J.W. and Hall, H.K. (1990) Adaptive acidification tolerance response of Salmonella typhimurium. J. Bacteriol., 172, 771-778 https://doi.org/10.1128/jb.172.2.771-778.1990
  40. Castro-Rosas, J. and Escartin, E.F. (1999) Incidence and germicide sensitivity of Salmonella typhi and Vibrio cholerae $O_1$ in alfalfa sprouts. J. Food Safety, 19, 137-146 https://doi.org/10.1111/j.1745-4565.1999.tb00240.x