The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Fusarium Species Associated with Corn Ear Rot

옥수수 이삭썩음병에 관여하는 Fusarium속균의 동정

  • Choi, Hyo-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Jung-Mi (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Jin-Hee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Hong, Sung-kee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Chun, Se-Chul (Department of Molecular Biotechnology, College of Life and Environmental Sciences, Konkuk University)
  • 최효원 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정미 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김진희 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 홍성기 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 천세철 (건국대학교 생명환경과학대학 분자생명공학과)
  • Published : 2009.12.31
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Abstract

In 2007, a total of 77 isolates of Fusarium spp. were obtained from ear rot symptoms of corns collected from 5 locations in Gangwon Province, Korea. The fungal isolates were identified based on their morphological features. Out of the isolates, fifteen isolates were identified as Fusarium verticillioides which formed microconidia in long chains on monophialides. Four isolates were identified as F. subglutinans which formed microconida only on false heads. Six isolates were identified as F. graminearum which produced red pigment in PDA culture. Besides these Fusarium species, F. napiform, F. nygamai, and F. oxysporum were identified from the rest isolates. To assess for genetic diversity of the isolates, a random amplified polymorphic DNA(RAPD) technique was carried out using URP primers. The results from the RAPD analysis showed that the isolates from corn were divided into 6 groups. These RAPD groups of the Fusarium species corresponded to morphological characters of the Fusarium species. The phylogenetic analysis of most isolates by DNA sequencing of EF-1$\alpha$ gene corresponded to morphological characters of the Fusarium species. The results of pathogenicity tests by two inoculation methods revealed that F. verticillioides, F. graminearum and F. subglutinans are strongly pathogenic to corn stalks.

Keywords

References

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