DOI QR코드

DOI QR Code

Identification of an entomopathogenic fungus, Nomuraea rileyi ANU101, infecting the beet armyworm, Spodoptera exigua, in Korea

국내 서식하는 파밤나방(Spodoptera exigua)에서 분리된 곤충병원성곰팡이 (Nomuraea rileyi ANU101)의 동정

  • Hwang, Ji-Hyeun (Department of Bioresource Sciences, Andong National University) ;
  • Park, Bok-Ri (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Geun-Seop (Department of Bioresource Sciences, Andong National University) ;
  • Lee, Sun-Gu (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
  • 황지현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박복리 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김근섭 (안동대학교 자연과학대학 생명자원과학과) ;
  • 이순구 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Published : 2009.12.31

Abstract

Several entomopathogenic fungi have been exploited to be developed into biological control agents in insect pest management. The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various crops, but not effectively controlled by commercial chemical pesticides due to its high insecticide resistance. A fungal isolate was isolated from S. exigua larvae collected from cabbage field in Andong, Korea. The fungus could be cultured in potato dextrose agar. Larvae of S. exigua injected with the cultured conidia showed a potent entomopathogenicity. To identify the fungus isolate, its internal transcribed space (ITS) and surrounding partial 18S/28S regions were sequenced. The ITS sequence was highly matched (99%) to that of Nomuraea rileyi. Morphological characters of its hyphae and conidia were well fit to those of known N. rileyi. This study reports the first record of an entomopathogenic fungus, N. rileyi, in Korea.

Keywords

References

  1. Amiri-Besheli, B., Khambay, B., Cameron, S., Deadman, M., Butt, T. M. 2000. Inter- and intra-specific variation in destruxin production by the insect pathogenic Metarhizium, and its significance to pathogenesis. Mycol Res 104:447-452. https://doi.org/10.1017/S095375629900146X
  2. Frappier, F., Ferron, P. and Pais, M. 1975. Chimie des champignons entomopathogenes - le beauvellide, nouveau cyclodepsipeptide isole d'un Beauveria tenella. Phytochemistry 14:2703-2705. https://doi.org/10.1016/0031-9422(75)85254-X
  3. Goh, H. G., Lee, S. G., Lee, B. P., Choi, K. M. and Kim, J. W. 1991.Simple mass-rearing of beet armyworm, Spodoptera exigua (Hbner) (Lepidoptera: Noctuidae), on an artificial diet. Kor. J. Appl. Entomol. 29:180-183.
  4. Goettel, M. S., Eilenberg, J. and Glare, T. 2005. Entomopathogenic fungi and their role in regulation of insect populations. In : Gilbert LI, Iatrou K, Gill SS, (eds), Molecular Insect Science, Elsevier, New York, pp 361-405.
  5. Hajek, A. E. and St. Leger, R. J. 1994. Interactions between fungal pathogens and insect hosts. Annu Rev Entomol 39:293-322. https://doi.org/10.1146/annurev.en.39.010194.001453
  6. Hamill, R. L., Higgens, C. E., Boaz, H. E. and Gorman, M. 1969. The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina. Tetrahed Lett 49:4255-4258.
  7. Jeffs, L. B. and Khachatourians, G. G. 1997. Toxic properties of Beauveria pigments on erythrocyte membranes. Toxicon 35:1351-1356. https://doi.org/10.1016/S0041-0101(97)00025-1
  8. Kim, Y., Lee, J., Kang, S. and Han, S. 1997. Variation in insecticide susceptibilities of the beet armyworm, Spodoptera exigua (Hbner): esterase and acetylcholinesterase activities. Kor J Appl Entomol 36: 172-178.
  9. Kim, Y., Lee, J., Kang, S. and Han, S. 1998. Age variation in insecticide susceptibility and biochemical changes of beet armyworm, Spodoptera exigua (Hbner). J Asia Pac Entomol 1: 109-113. https://doi.org/10.1016/S1226-8615(08)60012-6
  10. Kodaira, Y. 1961. Biochemical studies on the muscardine fungi in the silkworms, Bombyx mori. J Fac Text Sci Technol Sinshu Uni Sericult 5:1-68.
  11. Krasnoff, S. B. and Gupta, S. 1994. Identification of the antibiotic phomalactone from the entomopathogenic fungus Hirsutella thompsonii var. synnematosa. J Chem Ecol 20:293-302. https://doi.org/10.1007/BF02064437
  12. Krasnoff, S. B., Gupta, S., St. Leger, R. J., Renwick, J. A. and Roberts, D. W. 1991. Antifungal and insecticidal properties of efrapeptins: metabolites of the fungus Tolypocladium niveum. J Invertebr Pathol 58:180-188. https://doi.org/10.1016/0022-2011(91)90062-U
  13. Lacey, L. A. 1997. Manual of Techniques in Insect Pathology. pp. 156-167
  14. Matha, V., Weiser, J. and Olejnicek, J. 1988. The effect of tolypin in Tolypocladium niveum crude extract against mosquito and blackfly larvae in the laboratory. Folia Parasitol 35:381-383.
  15. Mazet, I. and Vey, A. 1995. Hirsutellin A, a toxic protein produced in vitro by Hirsutella thompsonii. Microbiol Reading 141: 1343-1348. https://doi.org/10.1099/13500872-141-6-1343
  16. Omoto, C. and McCoy, C. W. 1998. Toxicity of purified fungal toxin hirsutellin A to the citrus rust mite Phyllocoprura oleivora. J Invertebr Pathol 72:319-322. https://doi.org/10.1006/jipa.1998.4813
  17. Ovchinnikov, Y. A., Ivanov, V. T. and Mikhaleva, I. I. 1971. The synthesis and some properties of beauvericin. Tetrahed Lett 2:159-162.
  18. Quiot, J. M., Vey, A., Vago, C. and Pais, M. 1980. Action antivirale d'une mycotoxine. Etude d'une toxine de l'hyphomycete Metarhizium anisopliae (Metsch.) Sorok. en culture cellulaire. CR Acad Sci Ser D (Paris) 291:763-766.
  19. Roberts, D. W. and Hajek, A. E. 1992. Entomopathogenic fungi as bioinsecticides. In : Leatham GF, (ed), Frontiers in Industrial Mycology. Chapman and Hall, New York, pp 144-159.
  20. Srisukchayakul, P., Wiwat, C. and Pantuwatana, S. 2005. Studies on the pathogenesis of the local isolates of Nomuraea rileyi against Spodoptera litura. ScienceAsia 31: 273-276. https://doi.org/10.2306/scienceasia1513-1874.2005.31.273
  21. Suzuki, A., Kanaoka, M., Isogai, A., Murakpshi, S., Ichinoe, M. and Tamura, S. 1977. Bassianolide, a new insecticidal cyclodepsipeptide from Beauveria bassiana and Verticillium lecanii. Tetrahed Lett 25:2167-2170.
  22. Terry, B. J., Liu, W. C., Cianci, C. W., Proszynski, E., Fernandes, P., Bush, K. and Meyers, E. 1992. Inhibition of herpes simplex virus type 1 DNA polymerase by the natural product oosporein. J Antibiotics 45:286-288. https://doi.org/10.7164/antibiotics.45.286
  23. Vey, A., Hoagland, R. E. and Butt, T. M. 2001. Toxic metabolites of fungal biocontrol agents. In : Butt TM, Jackson C, Magan N, (eds), Fungal Biocontrol Agents, CABI Publishing, Wallingford, pp 311-346

Cited by

  1. Insecticidal Effect of an Entomopathogenic Fungus, Beauveria bassiana ANU1 to Spodoptera exigua and Plutella xylostella by Different Temperature and Humidity Conditions vol.19, pp.2, 2015, https://doi.org/10.7585/kjps.2015.19.2.125