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Antioxidant Activities of the Extract Fractions from Suaeda japonica

칠면초 분획물의 항산화 활성

  • Choi, Jong-Il (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Yeon-Joo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Song, Beom-Sok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Yoon, Yo-Han (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kwon, Joong-Ho (Dept. of Food Science & Technology, Kyungppok National University) ;
  • Chun, Soon-Sil (Dept. of Food & Nutrition, Sunchon National University) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 최종일 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김연주 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김재훈 (한국원자력연구원 정읍방사선과학연구소) ;
  • 송범석 (한국원자력연구원 정읍방사선과학연구소) ;
  • 윤요한 (한국원자력연구원 정읍방사선과학연구소) ;
  • 변명우 (한국원자력연구원 정읍방사선과학연구소) ;
  • 권중호 (경북대학교 식품공학과) ;
  • 전순실 (순천대학교 식품영양학과) ;
  • 이주운 (한국원자력연구원 정읍방사선과학연구소)
  • Published : 2009.02.28

Abstract

This study was designed to investigate the antioxidant effect of Suaeda japonica grown in Suncheon Bay. S. japonica was extracted using different solvents and the extracts were examined for their antioxidative activities with various methods. When the total phenolic contents were determined, the contents in the ethyl acetate, butanol and methanol extracts were 21.33, 17.31, and 2.33 mg/g, respectively. Fractions of butanol extract recorded the highest values of DPPH radical scavenging activity, $\beta$-carotene bleaching assay, and FRAP assay. The DPPH radical scavenging activities of butanol, ethyl acetate, methanol and water fractions were 77.46, 74.43, 47.99, and 27.70%, respectively. The FRAP value of butanol extract was 2.42 mM. But, the fraction of ethyl acetate extract was recorded the highest TBARS value. These results suggest that S. japonica, the specialty of Suncheon, could be a potential source of natural antioxidants.

칠면초(Suaeda japonica)를 식품 및 공중보건 산업에서 활용하기 위한 기초자료를 마련하기 위하여 항산화성을 측정해 보았다. 칠면초 분획 추출물의 폴리페놀 함량을 측정한 결과 ethyl acetate 추출물의 폴리페놀 함량이 21.33 mg/g으로 나타났고, 다음으로 butanol(17.31 mg/g), methanol(2.33 mg/g) 순이었으며, water 추출물에서는 폴리페놀이 검출되지 않았다. $\beta$-carotene bleaching assay를 측정한 결과 칠면초 butanol 추출물의 흡광도 감소 정도가 가장 적었고 다음으로 ethyl acetate, methanol 및 water 추출물 순으로 나타났다. 칠면초 추출물의 DPPH 라디칼 소거능을 측정한 결과 butanol, ethyl acetate, methanol 및 water 추출물 순으로 각각 77.46, 74.43, 47.99 및 27.70%로 나타났다. FRAP 측정결과 butanol 추출물의 FRAP value가 2.42 mM로 가장 높게 나타났다. 칠면초 추출물은 모두 무처리군보다 낮은 지질산패도(TBARS)를 나타내었으며, 특히 methanol 및 ethyl acetate 추출물이 높은 지질산패 저하능을 나타내었다. Butanol 추출물은 전자공여능, $\beta$-carotene bleaching assay 및 FRAP 실험에서 가장 높은 항산화 활성을 보여주었고, ethyl acetate 추출물은 폴리페놀 및 TBARS 측정 실험에서 높은 항산화 활성을 나타내어 칠면초 각각의 추출물에 다양한 항산화를 갖는 항산화제가 존재하는 것을 확인할 수 있었다.

Keywords

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