KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Anti-oxidant and Whitening Effects of Cell Lytic Enzyme-treated Lotus Leaf Extract

세포벽 분해효소 처리에 의한 연잎 추출물의 항산화 및 tyrosinase 저해 활성

  • Choi, Sun-Ju (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Kim, So-Young (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Lee, Sung-Chul (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Lee, Jin-Man (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Lee, In-Suk (Gimpo Agricultural Technical Center) ;
  • Jung, Moon-Yung (Gimpo Agricultural Technical Center) ;
  • Yang, Sam-Man (Gimpo Agricultural Technical Center) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
  • 최선주 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 김소영 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 이성철 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 이진만 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 이인숙 (김포시농업기술센터) ;
  • 정문영 (김포시농업기술센터) ;
  • 양삼만 (김포시농업기술센터) ;
  • 채희정 (호서대학교 식품생물공학과 및 식품기능안전연구센터)
  • Received : 2009.11.26
  • Accepted : 2009.12.22
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of cell lytic enzyme treatment on total phenolic content, antioxidant and antityrosinase activities of lotus leaf were investigated. The dried lotus leaves were hydroyzed by cell lytic enzymes such as Promozyme, Ceremix, Pectinex, Ultraflo, Celluclast, Pentopan, Tunicase, Viscozyme at their optimum pHs (pH 5-8) at $50^{\circ}C$ for 4 hrs. Depending on the enzymes used, total phenolic compounds content was measured as $1,079-1,476{\mu}g$/mL, and antioxidant activities and whitening activities were increased by 5~10% and 20%, respectively Among the tested hydrolytic enzymes, Promozyme (pullulanase) was selected as the most suitable enzyme for the extraction of total polyphenol from lotus leaf. The optimal dosage of Promozyme were found to be 1-2% (w/w). By Promozyme treatment, total phenolic compounds content of the lotus extract significantly increased compared to the extraction without enzyme treatment.

Keywords