The Korean Journal of Oral and Maxillofacial Pathology (대한구강악안면병리학회지)

Korean Academy of Oral and Maxillofacial Pathology (대한구강악안면병리학회)
권호 표지

Methanol Extract of Leaves from Cudrania tricuspidata Effects in HT-29 Colorectal Adenocarcinoma

꾸지뽕나무(Cudrania tricuspidata) 잎의 추출물이 대장암세포주에 미치는 영향

  • Kim, Mi-Sook (Jeollanam-do Institute of Health and Environment) ;
  • Kim ,In-Ae (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Ko, Young-Jong (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Jeong, Jin-An (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Kim, Ji-Eun (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Song, Byong-Jun (Jeollanam-do Institute of Health and Environment) ;
  • Lim, Won-Bong (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Lim, Hoi-Soon (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Park, Ji-Il (Department of Dental Hygiene, Gwangju Health College) ;
  • Kim, Seo-Yune (Department of Dental Hygiene Songwon College University) ;
  • Choi, Hong-Ran (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University) ;
  • Kim, Ok-Joon (Research Institute, Department of Oral Pathology, School of Dentistry, Chonnam National University)
  • 김미숙 (전남보건환경연구원) ;
  • 김인애 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 고영종 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 정진안 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 김지은 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 송병준 (전남보건환경연구원) ;
  • 임원봉 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 임회순 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 박지일 (광주보건대학 치위생과) ;
  • 김서연 (송원대학 치위생과) ;
  • 최홍란 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소) ;
  • 김옥준 (전남대학교 치의학전문대학원 구강병리학교실 및 치의학연구소)
  • Published : 2009.02.28

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Abstract

The phytochemicals of many plants suggest their potential use as dietary supplements in cancer chemoprevention and chemotherapy. In the present study, antitumor activity of Cudrania tricuspidata, a plant native to East Asia, was investigated. Cell growth inhibition of the extract on HT-29 colorectal adenocarcinoma using MTT colorimetric assay was determined. Apoptosis on HT-29 cells was performed by DNA fragmentation analysis. $PGE_2$ release was measured by enzyme immunoassay, because $PGE_2$ is a key protumorigenic prostanoid in many human cancers. For the ROS scavenging activity, ROS level was detected by laser scanning confocal microscope. It was found that methanol extract of leaves inhibits cell viability by inducing apoptosis as evidenced by DNA fragmentation. Stem bark decreases synthesis of $PGE_2$, inflammatory mediator. Fruits exhibited pronounced ROS scavenging activity. Taken together, these results suggest that Cudrania tricuspidata exerts growth inhibition and anti-oxidation on HT-29 cells through apoptosis, ROS scavenging respectively that it may have anti-cancer properties.

Keywords

References

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