Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Different of Total Phenolics and Flavonoids, Radical Scavenging Activities and Nitrite Scavenging Effects of Momordica charantia L. According to Cultivars

여주 품종별 폴리페놀, 플라보노이드 함량과 라디칼 소거활성 및 아질산염 소거능

  • Boo, Hee-Ock (Department of Biotechnology, College of Nature Science, Chosun Univ.) ;
  • Lee, Hyun-Hwa (Department of Biotechnology, College of Nature Science, Chosun Univ.) ;
  • Lee, Jang-Won (Institute of HealthScience, Jeonju Univ.) ;
  • Hwang, Sung-Jin (Department of Biology, College of Nature Science, Chonnam National Univ.) ;
  • Park, Sang-Un (Division of Plant Science & Resources, College of agriculture & Life Sciences, Chungnam National Univ.)
  • 부희옥 (조선대학교 자연과학대학 생물학과) ;
  • 이현화 (조선대학교 자연과학대학 생물학과) ;
  • 이장원 (전주대학교 건강과학종합연구소) ;
  • 황성진 (전남대학교 자연과학대학 생물학과) ;
  • 박상언 (충남대학교 농업생명과학대학 식물자원학부)
  • Published : 2009.02.28
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Abstract

This study was carried out in order to determine the biological activities such as antioxidant activities in nine cultivars of Momordica charantia L. (Korea, China, Japan and Philippine native cultivars). Their antioxidant activities were measured using DPPH free radical scavenging, ABTS cation radical scavenging, and Nitrite scavenging ability. The highest total polyphenol content (16.82 ${\mu}g/m{\ell}$) measured in the native Korea cultivar, and this value was 4.0 ${\mu}g/m{\ell}$ higher than that of the 'Verde Beuhas' cultivar (12.82 ${\mu}g/m{\ell}$). The 'Peacock' cultivar had the highest total flavonoid amount which was 4.38 ${\mu}g/m{\ell}$. The free radical scavenging activity using DPPH method was the highest in the native China cultivar ($RC_{50}$ = 102.6 ${\mu}g/m{\ell}$). ABTS cation radical scavenging activity according to cultivars was significantly higher in 'Peacock'. Nitrite scavenging ability showed the most remarkable effect at the pH 1.2, exhibited to 81.5${\sim}$86.9% by addition of ethanol extract 1${\mu}g/m{\ell}$ from Momordica charantia L. These results suggest that Momordica charantia L. had the potent biological activities, and that their activities exhibited differently depending on cultivars.

Keywords

References

  1. Basch E, Gabardi S and Ulbricht C. (2003). Bitter melon (Momordica charantia): a review of efficacy and safety. Americal Journal of Health-System Pharmacy. 60:356-359
  2. Blois MS. (1954). Antioxidant determination by the of a stable free radical. Nature. 26:1199-1204
  3. Choi YM, Kim MH, Shin JJ, Park JM and Lee JS. (2003). The antioxidant activities of the some commercial teas. The Korean Society of Food Science and Nutrition. 32:723-727 https://doi.org/10.3746/jkfn.2003.32.5.723
  4. Folin AD and Denis W. (1915). A colorimetric method for the determination of phenols (and phenol derivatives) in urine. Journal of Biological Chemistry. 22:305-308
  5. Frame AD, Rios-Olivares E, De Jesus L, Ortiz D, Pagan J and Mendez S. (1998). Plants from Puerto Rico with anti-Mycobacterium tuberculosis properties. Puerto Rico Health Science Journal. 17:243 - 252
  6. Greenblatt M, Mirvish S and So BT. (1971). Nitrosamine studies: induction of lung adenomas by concurrent administration of sodium nitrite and secondary amines in Swiss mice. Journal of National Cancer Institute. 46:1029-1034
  7. Grover JK and Yadav SP. (2004). Pharmacological actions and potential uses of Momordica charantia: a review. Journal of Ethnopharmacology. 93:123-132 https://doi.org/10.1016/j.jep.2004.03.035
  8. Hong TG, Lee YR, Yim MH and Hyun CN. (2004). Physiological functionality and nitrite scavenging ability of fermentation extracts from pine needles. The Korean Journal of Food Preservation. 11:94-99
  9. Joung YM, Park SJ, Lee KY, Lee JY, Suh JK, Hwang SY, Park KE and Kang MH. (2007). Antioxidative and antimicrobial activities of Lilium species extracts prepared from different aerial parts. Korean Journal of Food Science and Technology. 39:452-457
  10. Konishi T, Satsu H, Hatsugai Y, Aizawa K, Inakuma T, Nagata S, Sakuda SH, Nagasawa H and Shimizu M. (2004). Inhibitory effect of a bitter melon extract on the P-glycoprotein activity in intestinal Caco-2 cells. British Journal of Pharmacology. 143:379-387 https://doi.org/10.1038/sj.bjp.0705804
  11. Lee-Huang S, Huang P, Huang PL, Bourinbaiar AS, Chen HC and Kung HF. (1995). Inhibition of the integrase of human immunodeficiency virus (HIV) type 1 by anti-HIV plant proteins MAP30 and GAP31. Proceeding of the National Academy Sciences of USA. 92:8818-8822 https://doi.org/10.1073/pnas.92.19.8818
  12. Lee SJ, Chung MJ, Shin JH and Jung NJ. (2000). Effect of natural plant components on the nitrite-scavenging. Journal of Food Hygiene and Safety. 15:88-94
  13. Lee SO, Kim MJ, Kim DG and Choi HJ. (2005). Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. The Korean Society of Food Science and Nutrition. 34:139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  14. Lee YS. (2007). Antioxidant and physiologocal activity of extracts of Angelica dahurica leaves. The Korean Journal Food Preservation. 14:78-86
  15. Lim JA, Yun BW and Beak SH. (2007). Antioxidative activity and nitrite scavenging ability of methanol extract from Salvia plebeia R. Br. Korean Journal of Medicinal Crop Science. 15:183-188
  16. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 193:265-275 https://doi.org/10.1234/12345678
  17. Matkowski A and Piotrowska M. (2006). Antioxidant and free radical scavenging activities of some medicinal plants from the Lamiaceae. Fitoterapia. 77:346-53 https://doi.org/10.1016/j.fitote.2006.04.004
  18. Omoregbe RE, Ikuebe OM and Ihimire IG. (1996). Antimicrobial activity of some medicinal plants extracts on Escherichia coli, Salmonella paratyphi and Shigella dysenteriae. African Journal of Medical Science. 25:373-375
  19. Parkash A, Ng TB and Tso WW. (2002). Purification and characterization of charantin, a napin-like ribosome-inactivating peptide from bitter gourd (Momordica charantia) seeds. Journal of Peptide Research. 59:197-202 https://doi.org/10.1034/j.1399-3011.2002.00978.x
  20. Park Y, Boo HO, Park YL, Cho DH and Lee HH. (2007). Antioxidant activity of Momordica charantia L. extracts. Korean Journal of Medicinal Crop Science. 15:56-61
  21. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP and Rice-Evans C. (1995). Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Archives of Biochemistry and Biophysics. 322:339-46 https://doi.org/10.1006/abbi.1995.1473
  22. Shin JH, Lee JY, Ju JC, Lee SJ, Cho HS and Sung NJ. (2005). Chemical properties and nitrite scavenging ability of citron (Citrus junos). The Korean Society of Food Science and Nutrition. 34:496-502 https://doi.org/10.3746/jkfn.2005.34.4.496
  23. Srivastava Y, Venkatakrishna-Bhatt H and Verma Y. (1988). Effect of Momordica charantia L. pomous aqueous extract on cataractogenesis in murrin alloxan diabetics. Pharmacological Research Communications. 20:201-209
  24. Velioglu YS, Mazza G, Gao L and Oomah BD. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 46:4113-4117 https://doi.org/10.1021/jf9801973
  25. Vikrant V, Grover JK, Tandon N, Rathi SS and Gupta N. (2001). Treatment with extracts of Momordica charantia and Eugenia jambolana prevents hyperglycemia and hyperinsulinemia in fructose fed rats. Journal of Ethnopharmacology. 76:139-43 https://doi.org/10.1016/S0378-8741(01)00218-5
  26. Virdi J, Sivakami S, Shahani S, Suthar AC, Banavalikar MM and Biyani MK. (2003). Antihyperglycemic effects of three extracts from Momordica charantia. Journal of Ethnopharmacology. 88:107-111 https://doi.org/10.1016/S0378-8741(03)00184-3
  27. Wang M, Li J, Rangarajan M, Shao Y, Zhu N, LaVoie EJ, Huang TC and Ho CT. (1998). Antioxidative phenolic compound from sage (Salvia officinalis). Journal of Agricultural and Food Chemistry. 46:4869-4873 https://doi.org/10.1021/jf980614b