Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Immuno Modulatory Activities of Rubus coreanus Miquel Extracts by Nano-encapsulation Process

나노 입자화 한 복분자 저온 추출물의 면역 활성 증진

  • Han, Jae-Gun (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kim, Young (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kim, Jin-Chul (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
  • 한재건 (강원대학교 BT특성화학부대학) ;
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 정향숙 (강원대학교 BT특성화학부대학) ;
  • 김영 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 김진철 (강원대학교 BT특성화학부대학) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Published : 2009.02.28
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Abstract

This study was performed to improve immune activities of Rubus coreanus Miquel by encapsulation of nanoparticles. Immuno-activities of R. coreanus were investigated through aqueous extracts associated with process of water at $60^{\circ}C$. It showed high promotion of human B and T cells growth about 50%, compared to the case of other conditions. The secretion of IL-6 and TNF-${\alpha}$ was also enhanced as $2.44{\times}10^{-4}$pg/cell and $1.94{\times}10^{-4}$pg/cell, results by adding nano samples. NK cell activation was improved up to 29% higher than the conventional extraction process. The secretion of NO from macrophage showed 14.9 ${\mu}M$ on the nano-encapsulation process extracts, which was higher than others. The size of nanoparticles was in the range of 50${\sim}$300 nm, which can effect the penetration into the cells. It was clearly observed by real time confocal microscope.

Keywords

References

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