Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Protective Effect of Vitis amurensis Stems and Leaves Extract on Hydrogen Peroxide-induced Oxidative Neuronal Cell Damage in Cultured Neurons

과산화수소수로 유도된 배양 뇌신경세포손상에 대한 왕머루 잎과 줄기 추출물의 보호효과

  • Kim, Joo-Youn (College of Veterinary Medicine, Chungbuk National University) ;
  • Ju, Hyun-Soo (College of Veterinary Medicine, Chungbuk National University) ;
  • Ban, Ju-Yeon (Department of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Song, Kyung-Sik (College of Agriculture and Life-Sciences, Kyungpook National University) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Seong, Yeon-Hee (College of Veterinary Medicine, Chungbuk National University)
  • 김주연 (충북대학교 수의과대학) ;
  • 주현수 (충북대학교 수의과대학) ;
  • 반주연 (경희대학교 의과대학) ;
  • 송경식 (경북대학교 농업생명과학대학) ;
  • 배기환 (충남대학교 약학대학) ;
  • 성연희 (충북대학교 수의과대학)
  • Published : 2009.02.28
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Abstract

Vitis amurensis (VA; Vitaceae) has long been used in oriental herbal medicine. It has been reported that roots and seeds of VA have anti-inflammatory and antioxidant effects. In the present study, the protective effect of ethanol extract from stems and leaves of VA on hydrogen peroxide (${H_2}{O_2}$) (100 ${\mu}M$)-induced neuronal cell damage was examined in primary cultured rat cortical neurons. VA (10-100 ${\mu}g$/ml) concentration-dependently inhibited ${H_2}{O_2}$-induced apoptotic neuronal cell death measured by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. VA inhibited ${H_2}{O_2}$-induced elevation of intracellular $Ca^{2+}$ concentration (${[Ca^{2+}]}_i$) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Pretreatment of VA also prevented glutamate release into medium induced by 100 ${\mu}M$ ${H_2}{O_2}$, which was measured by HPLC. These results suggest that VA showed a neuroprotective effect on ${H_2}{O_2}$-induced neuronal cell death by interfering with ${H_2}{O_2}$-induced elevation of ${[Ca^{2+}]}_i$, glutamate release, and ROS generation. This has a significant meaning of finding a new pharmacological activity of stems and leaves of VA in the CNS.

Keywords

References

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