Antimicrobial Activity of Methanol Extracts from Cudrania tricuspidata Bureau according to the Parts Harvested and Time

수확부위 및 시기에 따른 꾸지뽕나무 메탄올 추출물의 항균성

  • Choi, So-Ra (Medicinal Plants Research Institute, Jeollabukdo ARES) ;
  • You, Dong-Hyun (Medicinal Plants Research Institute, Jeollabukdo ARES) ;
  • Kim, Jong-Yeob (Medicinal Plants Research Institute, Jeollabukdo ARES) ;
  • Park, Chun-Bong (Medicinal Plants Research Institute, Jeollabukdo ARES) ;
  • Kim, Dae-Hyang (Medicinal Plants Research Institute, Jeollabukdo ARES) ;
  • Ryu, Jeong (Jeollabukdo Agricultural Research and Extension Services) ;
  • Choi, Dong-Geun (Division of Biological Resources Science, Collage of Agriculture & Life Science, Chonbuk National University) ;
  • Park, Hyun-Mi (Medicinal Plants Research Institute, Jeollabukdo ARES)
  • 최소라 (전라북도농업기술원 약초연구소) ;
  • 유동현 (전라북도농업기술원 약초연구소) ;
  • 김종엽 (전라북도농업기술원 약초연구소) ;
  • 박춘봉 (전라북도농업기술원 약초연구소) ;
  • 김대향 (전라북도농업기술원 약초연구소) ;
  • 류정 (전라북도농업기술원) ;
  • 최동근 (전북대학교 농업생명과학 생물자원과학부) ;
  • 박현미 (전라북도농업기술원 약초연구소)
  • Published : 2009.10.30

Abstract

To evaluate the availability of Cudrania tricuspidata Bureau as a natural source of antimicrobials, the antimicrobial activity of methanol extracts of harvested parts was investigated using the paper disc diffusion method. The extracts from leaves and root bark had broad antimicrobial activity against various bacteria, including Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Enterobacter aerogenes, Enterococcus faecalis, Salmonella choleraesuis subsp. choleraesuis, Vibrio vulnificus, and Pseudomonas aeruginosa and inhibited Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes, agents of food poisoning especially well. The extract from ripe fruit had a very high antimicrobial activity against Staphylococcus aureus with a 20.2 mm of clear zone at 50 mg/mL sample concentration. These results indicated that Cudrania tricuspidata could be used as new source for developing natural antimicrobial agents.

Keywords

References

  1. Choi I, Cho JY and Lim SC. (2006). Antimicrobial activity of medicinal herbs against Staphylococcus aureus. Korean Journal of Plant Resources. 19:491-496
  2. Choi SR, You DH, Kim JY, Park CB, Kim DH and Ryu J. (2009). Antioxidant activity of methanol extracts from Cudrania tricuspidata Bureau according to harvesting parts and time. Korean Journal of Medicinal Crop Science. 17:115-120
  3. Choi SR, You DH, Kim JY, Park CB, Ryu J, Kim DH and Eun JS. (2008). Antioxidant and antimicrobial activities of Artemisia capillaris Thunberg. Korean Journal of Medicinal Crop Science. 16:112-117
  4. Chon IJ, Lee SW, Cha JH, Han JH and Whang WK. (2005). Anti-oxidant compounds of Cudrania tricuspidata leaves. Yakhak Hoeji. 49:416-421
  5. Han JS and Shin DH. (1994). Antimicrobial effect of each solvent fraction of Morus alba Linne, Sophora flavescens AITON on Listeria monocytogenes. Korean Society of Food Science and Technology. 26:539-544
  6. Han SH, Woo NRY, Lee SD and Kang MH. (2006). Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean Journal of Medicinal Crop Science. 14:49-55
  7. Kim SH, Kim NJ, Choi JS and Park JC. (1993). Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau. Journal of the Korean Society of Food Science and Nutrition. 22:68-72
  8. Kim YC, Sohn DH and Kim HS. (2008). Antibacterial compounds of the root barks of Cudrania tricuspidata. Korean Journal of Pharmacognosy. 39:246-248
  9. Lee BW, Kang NS and Park KH. (2004). Isolation of antibacterial prenylated flavonoids from Cudrania tricuspidata. Journal of the Korean Society for Applied Biological Chemistry. 47:270-273
  10. Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M and Yoo ID. (1996). Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochemistry. 41:213-216 https://doi.org/10.1016/0031-9422(95)00609-5
  11. Lee IK, Song KS, Kim CJ, Kim HM, Oh GT and Yoo ID. (1994). Tumor cell growth inhibition and antioxidative activity of flavonoids from the stem bark of Cudrania tricuspidata. Journal of the Korean Society of Agricultural Chemistry and Biotechnology. 37:105-109
  12. Lee SJ. (2002). Antioxidative activity of flavonoid compounds from Cudrania tricuspidata root bark. Master Thesis, Jungang University. Seoul, Korea
  13. Oh DH, Ham SS, Park BK, Ahn C and Yu JY. (1998). Antimicrobial activities of natural medicinal herbs on the food spoilage or foodborne disease microorganisms. Korean Society of Food Science and Technology. 30:957-963
  14. Paek DH. (2007). Screening of the natural plant extracts for the antimicrobial activity on dental pathogens. The Korean Journal of Microbiology. 43:227-231
  15. Tian YH, Kim HC, Cui JM and Kim YC. (2005). Hepatoprotective constituents of Cudrania tricuspidata. Archives of Pharmacal Research. 28:44-48 https://doi.org/10.1007/BF02975134
  16. Yang MS, Ha YL, Nam SH, Choi SU and Jang DS. (1995). Screening of domestic plant with antibacterial activity. Journal of the Korean Society of Agricultural Chemistry and Biotechnology. 38:584-589
  17. Yoo ID. (1995). Development of screening system and discovery of bioactive substances. Korea Research Institute of Bioscience & Biotechnology. Daejeon, Korea. p. 9-27