Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antimicrobial Activities of Extracts of Prunus mume by Sugar

매실 당침출액의 항균활성

  • Ko, Myung-Soo (Department of Food Science and Biotechnology, Dongnam Health College) ;
  • Yang, Jong-Beom (Department of Food Science and Biotechnology, Dongnam Health College)
  • 고명수 (동남보건대학 식품생명과학과) ;
  • 양종범 (동남보건대학 식품생명과학과)
  • Published : 2009.10.30
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Abstract

The antimicrobial activities of extracts of Prunus mume by sugar against food poisoning bacteria, and the effects of heat and pH treatment on these antimicrobial activities, were investigated. The level of total solids, pH, and acidity of P. mume extracts were 55.08% (w/w), pH 3.1, and 1.52%, respectively. P. mume extracts showed the strongest antimicrobial activity against Vibrio parahaemolyticus, among the bacteria tested. P. mume extracts significantly inhibited the growth of V. parahaemolyticus, Bacillus cereus, and Staphylococcus aureus at levels of 1-2% (w/v) of extracts in media. The antimicrobial activities of P. mume extracts were neither affected by heating at $65-125^{\circ}C$ for 30 minutes, nor by neutralization of extract to pH 7.0.

매실에 설탕을 가한 후 삼투압의 원리를 이용하여 제조한 매실 침출액의 총고형분은 55.08%였고, pH는 3.1이었으며, 산도는 구연산으로서 1.52%였다. 이 매실 침출액의 식중독세균에 대한 항균활성을 조사한 결과, 공시된 모든 균주 중에서Vibrio parahaemolyticus에 대해 31.2 mm의 저해환을 보여 이 균에 대한 매실 침출액의 항균활성이 가장 강하였고, 다음으로 Bacillus cereus, Staphylococcus aureus 및 Salmonella typhymurium의 순으로 활성이 강하였다. 매실 침출액은 공시된 모든 시험균주에 대해 생육저해효과가 있는 것으로 나타났다. 특히 Vibrio parahaemolyticus에 대해서는 0.25%에서 생육이 억제되어 생육저해효과가 가장 높은 것으로 나타났고, 이 균에 대한 MIC도 1.0%로 가장 낮았다. Bacillus cereus와 Staphylococcus aureus에 대해서는 2%이상의 농도에서는 생육이 완전히 억제되었으나, 1.0%이하의 농도에서는 생육이 촉진되는 것으로 나타나 매실 침출액 중의 미지의 성분이 이들의 주요 영양원으로 이용되었음을 알 수 있었다. 매실 침출액의 열 및 pH 안정성을 측정한 결과, $65{\sim}125^{\circ}C$의 온도범위에서 열에 안정하였고, pH 7로의 중화에 따른 영향을 받지 않았다.

Keywords

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