Journal of Electrical Engineering and Technology

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지
DOI QR코드

DOI QR Code

Electrochemical Detection of Self-Assembled Viologen Modified Electrode as Mediator of Glucose Sensor

  • Lee, Dong-Yun (Dept. of Electrical Engineering & NTRC, Dong-A University) ;
  • Choi, Won-Suk (Dept. of Electrical Engineering & NTRC, Dong-A University) ;
  • Park, Sang-Hyun (Dept. of Electrical Engineering & NTRC, Dong-A University) ;
  • Kwon, Young-Soo (Dept. of Electrical Engineering & NTRC, Dong-A University)
  • Published : 2009.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

An amperometric glucose biosensor has been developed using viologen derivatives as a charge transfer mediator between a glucose oxidase (GOD) and a gold electrode. A highly stable self-assembled monolayer (SAM) of thiol-based viologen was immobilized onto the gold electrode of a quartz crystal microbalance (QCM) and GOD was immobilized onto the viologen modified electrode. This biosensor response to glucose was evaluated amperometrically in the potential of -300mV. Upon immobilization of the glucose oxidase onto the viologen modified electrode, the biosensor showed rapid response towards glucose. Experimental conditions influencing the biosensor performance, such as pH potential, were optimized and assessed. This biosensor offered excellent electrochemical responses for glucose concentration below ${\mu}$ mol level with high sensitivity and selectivity and short response time. The levels of the RSDs (<5%) for the entire analyses reflected the highly reproducible sensor performance. A linear calibration range between the current and the glucose concentration was obtained up to $4.5{\times}10^{-4}M$. The detection limit was determined to be $3.0{\times}10^{-6}M$.

Keywords

References

  1. L. Clark and C. Lyons, 'Electrode systems for continuous monitoring cardiovascular surgεry', Ann. NY Acad. Sci. 102 (1962) 29 https://doi.org/10.1111/j.1749-6632.1962.tb13623.x
  2. S. J. Updike and J. P. Hicks, 'The enzyme electrode', Nature, 214 (1967) 986 https://doi.org/10.1038/214986a0
  3. N. Nakamura, H.-X. Huang, D.-J. Qian and J Miyake, 'Quartz Crystal Microbalance and Electrochemical Studies on a Viologen Thiol Incorporated in Phospholipid Self-Assembled Monolayers', Langmuir, 18 (2002) 5804 https://doi.org/10.1021/la020070e
  4. D.-Y. Lee, A.K.M. Kafi, S.-H. Park, D.-J. Qian, Y. -S. Kwon, 'Influence of Anions on Electrochemical Redox Reaction and Electrical Property using Violgen Derivative', Jpn. J. Appl. Phys. 45 (4B) (2006) 3772 https://doi.org/10.1143/JJAP.45.3772
  5. D.-Y. Lee, A.K.M. Kafi, S.-H. Park, Y. -S. Kwon, 'Charge Transfer Property of Self-Assembled Viologen Derivative by Electrochemical Quartz Crystal Microbalance Response', J. Nanosci. Nanotechnol., 6 (2006) 3657 https://doi.org/10.1166/jnn.2006.076
  6. A.K.M. Kafi, D.-Y. Lee, S.-H. Park, Y. -S. Kwon, 'Development of a peroxide biosensor made of a thiolated-viologen and hemoglobin-modified gold electrode', Microchem. J., 85 (2007) 308 https://doi.org/10.1016/j.microc.2006.07.007
  7. J.-Y. Ock, H.-K. Shin, Y. -S. Kwon, J. Miyake, 'Study on the electrochemical behavior of thε viologen monolayεrs by different chεmical structure', Colloid. Surface. A, 257 (2005) 351 https://doi.org/10.1016/j.colsurfa.2004.08.079
  8. D.-Y. Lee, W.-S. Choi, A.K.M. Kafi, S.-H. Park, Y. -S. Kwon, 'Immobilization, Hybridization and Amperometric Detection of DNA Sensor Using Quartz Crystal Microbalance', J. Nanosci. Nanotechnol. , 8 (2008) 4553 https://doi.org/10.1166/jnn.2008.IC21
  9. S. Hu, C.-C. Liu, 'Development of a hypoxanthine biosensor based on immobilized xanthine oxidase chemically modified electrode', Electroanal., 9 (1997) 372 https://doi.org/10.1002/elan.1140090504
  10. M. E. Ghica, Christopher M. A. Brett, 'A glucose biosensor using methyl viologen redox mεdiator on carbon film electrodes', Anal. Chim. Acta, 532 (2005) 145 https://doi.org/10.1016/j.aca.2004.10.058
  11. A.K.M. Kafi, F. Yin, H.-K. Shin, Y.-S. Kwon, 'Hydrogen peroxide biosensor based on DNA-Hb modified gold electrode', Thin Solid Films, 499 (2006) 420 https://doi.org/10.1016/j.tsf.2005.06.073
  12. A. I. Yaropolov, S. V. Shleev, O. V. Morozova, E. A. Zaitseva, G. Marko-Varga, J. Enmeus, L. Gorton, 'An Amperomεtric Biosensor Based on Laccase Immobilized in Polymer Matrices for Determining Phenolic Compounds', J. Anal. Chem. , 60 (2005) 553 https://doi.org/10.1007/s10809-005-0138-2

Cited by

  1. Electrochemical detection of hydrogen peroxide via hemoglobin–DNA/pyterpy-modified gold electrode vol.9, pp.4, 2009, https://doi.org/10.1016/j.cap.2009.06.048
  2. Self-assembled monolayers (SAMs) for electrochemical sensing vol.15, pp.7-8, 2011, https://doi.org/10.1007/s10008-011-1493-6
  3. A Microfluidic Electrochemical Sensor for Detecting the Very Low Concentration Endocrine Disruptor with Self Assembled Monolayer and Preconcentration Technique vol.65, pp.4, 2016, https://doi.org/10.5370/KIEE.2016.65.4.628