Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
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Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP

종 특이 PCR과 PCR-RFLP를 이용한 웅담과 기타 담류의 감별 방법

  • Kwon, Ki-Rok (College of Oriental Medicine, Sangji University) ;
  • Baek, Seung-Il (College of Oriental Medicine, Sangji University) ;
  • Choi, Suk-Ho (Division of Animal Science and Life Resources, Sangji University)
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 백승일 (상지대학교 한의과대학 침구학교실) ;
  • 최석호 (상지대학교 생명자원과학대학, 동물생명자원학부)
  • Published : 2009.03.30
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Abstract

Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria. Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the speciesspecific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII. Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.

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References

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