Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
권호 표지

Possibility of Gene Flow and Unintended Escape from Leaf-folder (Cnaphalocrocis medinalis) Resistant Rice

혹명나방저항성벼의 잡초화 및 유전자 이동 가능성

Lee, In-Young;Park, Jae-Eup;Moon, Beong-Chul;Suh, Seok-Cheol;Shin, Kong-Sik;Woo, Mi-Ok;Kweon, Soon-Jong
이인용;박재읍;문병철;서석철;신공식;우미옥;권순종

  • Published : 20090300
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Abstract

This study was carried out to investigate the possibility of weediness and gene flow from leaf-folder(Cnaphalocrocis medinalis) resistant rice ('06 : Event C7-1-9-1. '07 : C7-1-9-1-1, '08 : C7-1-9-1-1-1). The leaf-folder resistant rice was germinated 100% at 1 month after bury into soil and was showed 0% after 3 month. The shattered rice seeds during harvest were germinated 27% in Chuchungbyeo, 2.7% in GM rice and 3.3% in Nakdongbyeo under the field condition. However, germination pattern was different according to overwinter condition. When GM rice was unintentional escape in outdoor of the leaf-folder resistant rice can be controlled with glyphosate and paraquat of non-selective herbicides. In order to investigate gene flow from the leaf-folder resistant rice, Nakdongbyeo and weedy rice were planted with difference distance. Degree of survival was higher at near planting Nakdongbyeo from the leaf-folder resistant rice. The detection of gene flow from the leaf-folder resistant rice was done using a lateral flow strip test kit. Gene of the leaf-folder resistant rice can be detected by lateral flow strip test. But showed no survival plant from weedy rice because of differential stage of heading. Weed flora in the culture field of leaf-folder resistant rice were very similar with culture field of non GM rice.

혹명나방저항성벼('06년 Event C7-1-9-1, '07년 Event C7-1-9-1-1, '08년 Event C7-1-9-1-1-1)의 잡초화 및 유전자 이동 가능성을 조사하기 위하여 여러 가지 시험을 수행하였다. 토양 중에 매몰한 후 1개월 경과된 모든 종자는 100% 발아하였으나, 3개월 이후에는 모두 발아율이 0%이었다. 수확과정에서 탈립된 종자 중 자연노출조건에서 추청벼는 27% 발아한 반면에 GM벼는 2.7%, 낙동벼는 3.3% 발아되어 월동조건에 따라 발아정도가 상이하였으나, 물에 떨어진 종자는 익년도에 발아가 되지 않았다. 비의도적으로 일출된 혹명나방저항성벼는 글리포세이트액제나 패라쾃디클로라이드액제와 같은 비선택성 제초제로 방제 가능하였다. 혹명나방저항성벼와 재배벼인 낙동벼 간의 유전자 이동여부는 거리별로 수확한 후 바스타액제 처리 결과, 근접거리가 가까운 벼에서 바스타에 생존하는 비율이 높아 화분교잡에 의한 유전자이동 비율이 8.6~11.6%를 보였다. 이러한 유전자 이동여부는 test kit를 이용하여 유전자 함유여부를 최종 확인할 수 있었다. 저항성 벼와 잡초성벼간의 유전자 이동시험에서 바스타 처리시 생존하는 개체가 없었는데 이러한 결과는 출수기가 달랐기 때문으로 사료된다. 시험포장에 발생된 잡초는 일년생 및 다년생잡초가 고르게 분포되었으며, 혹명나방저항성벼와 인근 포장이 잡초발생 양상이 유사하였다.

Keywords

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