Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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A Novel Antifungal Analog Peptide Derived from Protaetiamycine

  • Lee, Juneyoung (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Hong, Hyun Joo (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Jin-Kyoung (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Hwang, Jae-Sam (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Yangmee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Lee, Dong Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2009.09.07
  • Accepted : 2009.09.22
  • Published : 2009.11.30
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Abstract

Previously, the 9-mer analog peptides, 9Pbw2 and 9Pbw4, were designed based on a defensin-like peptide, protaetiamycine isolated from Protaetia brevitarsis. In this study, antifungal effects of the analog peptides were investigated. The antifungal susceptibility testing exhibited that 9Pbw4 contained more potent antifungal activities than 9Pbw2. A PI influx assay confirmed the effects of the analog peptides and demonstrated that the peptides exerted their activity by a membrane-active mechanism, in an energy-independent manner. As the noteworthy potency of 9Pbw4, the mechanism(s) of 9Pbw4 were further investigated. The membrane studies, using rhodamine-labeled giant unilamellar vesicle (GUV) and fluorescein isothiocyanate (FITC)-dextran loaded liposome, suggested that the membrane-active mechanism of 9Pbw4 could have originated from the pore-forming action and the radii of pores was presumed to be anywhere from 1.8 nm to 3.3 nm. These results were confirmed by 3D-flow cytometric contour-plot analysis. The present study suggests a potential of 9Pbw4 as a novel antifungal peptide.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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