Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Micropropagation of Paprika (Capsicum annuum) and Its Subsequent Performance in Greenhouse Cultivation

파프리카(Capsicum annuum)의 미세번식과 이후 온실 재배에서의 생산성

Song, Ju-Yeon;Sivanesan, Iyyakkannu;An, Chul-Geon;Jeong, Byoung-Ryong
송주연;;안철근;정병룡

  • Published : 2009.06.30
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Abstract

Paprika (Capsicum annuum) is one of the important fruit vegetable crops and there is a big demand in the world fresh market. Conventionally the species is propagated mainly by seeds, but seeds of paprika are expensive, especially in Korea, and a burden to growers. Therefore, a simple micropropagation system was developed for paprika 'Ferrari' using nodal explants. Also, plant growth and fruit productivity were investigated by comparing micropropagated plants (MPP) vs. seed propagated plants (SPP). In the micropropagated plants, seeds were germinated on the 1/2 MS in a dark condition, and shoot tip explants were taken and cultured on the MS medium. Elongated shoots were cut into multiple single nodal cuttings and then were cultured on the fresh MS for shoot growth and rooting. Well-rooted plants were acclimatized in a greenhouse. In the seed propagated plant, seeds were germinated in rockwool cubes and then seedlings were transplanted in rockwool slabs after true leaves appeared. In the comparison of greenhouse performance of SPP and MPP, no. of fruits from MPP was 49.8% higher than that of SPP, though its plant height and main stem length were smaller. Therefore, micropropagated plants can be expected to be used as alternative propagules and to increase grower’s income by decreasing cost on propagules.

파프리카(Capsicum annuum)는 중요한 신선 과채류 작물 중 하나로 세계적으로 수요가 증가하고 있다. 일반적으로 파프리카는 온실에서 종자로 번식하나 종자값이 비싸 농가에 부담이 가중되고 있다. 따라서 본 연구에서는 파프리카의 마디배양을 통한 대량번식 시스템을 개발하고, 수확된 조직배양묘와 일반묘의 생육특성과 과실의 수 및 무게를 조사하였다. 조직배양묘는 파프리카의 종자를 1/2 MS배지에서 암발아시켰다. 발아된 유식물체를 경단을 포함한 식물체를 MS배지에서 배양하고, 생장한 식물체의 마디를 잘라 새로운 배지에 옮겨 신초생장과 발근을 유도한 후, 온실에서 순화하였다. 일반묘는 종자를 암면플러그에서 발아시킨 후 본엽이 완전히 전개된 후 암면슬래브에 이식하여 조직배양묘와 일반묘의 번식 이후 생산성을 비교하였다. 일반묘와 조직배양묘의 생산성 검증에서는 조직배양묘가 일반묘에 대해 초장과 주경장의 길이는 작았으나총 과실의 수는 약 49.8%가 많았다. 따라서 조직배양을 이용한 묘를 농가에 보급함으로서 비싼 종자를 이용한 번식방법을 대신할 수 있게 되고, 번식비용을 감소시킴으로써 재배농가에 소득향상이 기대된다.

Keywords

References

  1. Agrawal, S., N. Chandra, and S.L. Kothari. 1998. Shoot-tip culture of pepper for micropropagation. Curr. Sci. 57:134-1349
  2. Ahmad, N. and M. Anis. 2005. In vitro mass propagation of Cucumis sativus L. from nodal segments. Turk. J. Bot. 29:237-240
  3. Anilkumar, M. and A.S. Nair. 2004. Multiple shoot induction in Capsicum annuum L. cv. Early California Wonder. Plant Cell Biotech. Mol. Biol. 5:95-100
  4. Bae, J.H. 2000. High quality-sweet pepper technique. Korea Hydroponic Society Symposium, Spring 2000. p. 55
  5. Bhatt, I.D. and U. Dhar. 2000. Micropropagation of Indian wild strawberry. Plant Cell, Tissue and Organ Culture 60:83-88 https://doi.org/10.1023/A:1006471815566
  6. Cho, Y.C., J.D. Moon, J.Y. Song, and B.R Jeong. 2007. Development of micropropagation methods of shallot (Allium cepa var. ascalonicum Backer). Kor. J. Hor. Sci. Techol. 25:322-327
  7. Choi, K.Y., M.J. Kang, Y.B. Lee, S.O. Yoo, and J.H. Bae. 2001. Development of optimum nutrient solution for sweet pepper substrate culture in closed system. J. Kor. Soc. Hort. Sci. 45:513-518
  8. Choi, S.K. 2003. In vitro propagation using shoot tip culture of Curcuma lnga L. Kor. J. Crop Sci. 48:438-441
  9. Daood, H.G., M. Vinkler, F. Márkush, E. Hebshi, and P. Biacs. 1996. Antioxidant vitamin content of spice red pepper (paprika) as affected by technological and varietal factors. Food Chem. 55:365-372 https://doi.org/10.1016/0308-8146(95)00136-0
  10. Fari, M., A. Szasz, J. Mityko, I. Nagy, M. Csanyi, and A. Andrasfalvy. 1992. Induced organogenesis via the seedling decapitation method (SDM) in three solanaceous vegetable species. Capsicum Newsletter 243-248
  11. Izadpanah, M. and M. Khosh-Khui. 1992. Comparisons of in vitro propagation of tomato cultivars. Iran Agric. Res. 8:37-47
  12. Kang, K.Y., T.K. Yun, and J.S. Choi. 1981. Effect of virus infection on the growth and the rate of photosynthesis of strawberry plants. J. Kor. Soc. Hort. Sci. 22:80-85
  13. Lee, E.M. and Y.B. Lee. 1996. Field trials of micropropagated strawberry plants. Kor. J. Plant Biotechnol. 23:297-282
  14. Maria, J.P. 2006. Conservation of Vaccinium cylindraceum Smith (Ericaceae) by using micropropatation using seedling nodal explants. In Vitro Cell Dev. Biol. Plant 42:65-68
  15. Ma´rkus, F., H.G. Daood, J. Kapita´ny, and P.A. Biacs. 1999. Change in the carotenoid and antioxidant content of spice red pepper (paprika) as a function of ripening and some technological factors. J. Agric. Food Chem. 47:100-107 https://doi.org/10.1021/jf980485z