Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
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Organic Nutrition and Gene Expression in Different Tissues of Chinese Cabbage

Mun, Myeong-Il;Xu, Ming;Park, Young-Doo;Hur, Yoon-Kang

  • Published : 20090400
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Abstract

To evaluate the nutritional value of Chinese cabbage, carbohydrate, vitamin C, carotenoid and free amino acid levels were measured in six different tissues (leaf and midrib of outer leaves, leaf and midrib of middle leaves, and leaf and midrib of inner leaves) of fully-headed Chinese cabbage. Gene expression associated with the biosynthesis of these compounds was also examined. High levels of starch and vitamin C were detected in the leaves while midrib parts contained low levels. The outer leaf parts contain the lowest level of glucose and sucrose, in contrast to high levels observed in inner parts. However, the outer leaves contained the highest content of carotenoids. While Chinese cabbage contains high levels of Glu, Ala, Asp, and Arg, the levels of most essential amino acids are very low. Transcript levels tested in this study did not correspond to nutrient levels, implying biosynthesis of organic nutrients lacks regulation at the transcriptional level. These results collectively show that although the outer leaves of Chinese cabbage contain more health compounds such as vitamins and carotenoid, the inner leaves are more nutritious.

Keywords

References

  1. Bartley, G.E. and P.A. Scolnik. 1995. Plant carotenoid: pigments for photoprotection, visual attraction, and human health. Plant Cell 7:1027-1038 https://doi.org/10.1105/tpc.7.7.1027
  2. Bone, R.A., J.T. Landrum, L.H. Guerra, and C.A. Ruiz. 2003. Lutein and zeaxanthin dietary supplements raise macular pigment density and serum concentrations of these carotenoids in humans. J. Nutr. 133:992-998
  3. Coruzzi, G. and R. Last. 2000. Amino acids, P. 358-410. In: B. Buchanan et al. (ed.). Biochemistry and Molecular Biology of Plants. Plant Physiol., Rockville, MD
  4. Dangnelie, G., I.S. Zorge, and T.M. McDonald. 2000. Lutein improves visual function in some patients with retinal degeneration: a pilot study via the internet. Optometry 71:147-164
  5. Davison, P.A., C.N. Hunter, and P. Horton. 2002. Overexpression of beta-carotene hydroxylase enhances stress tolerance in Arabidopsis. Nature 418:2003-2006
  6. Demmig-Adams, B., A.M. Gilmore, and W.W. III Adams. 1996. In vivo functions of carotenoids in higher plants. FASEB J. 10:403-412
  7. Demmig-Adams, B. and W.W. III Adams. 2002. Antioxidants in photosynthesis and human nutrition. Science 298:2149-2153 https://doi.org/10.1126/science.1078002
  8. De Tullio, M.C. and O. Arrigoni. 2004. Hopes, disillusions and more hopes from vitamin C. Cell. Mol. Life Sci. 61:209-219 https://doi.org/10.1007/s00018-003-3203-8
  9. Endo, Y., R. Usuki, and T. Kaneda. 1985. Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oils in the dark. 1. Comparison of the inhibitory effects. JAOCS 62:1375-1378 https://doi.org/10.1007/BF02545962
  10. Forde, B.G. and P.J. Lea. 2007. Glutamate in plants: metabolism, regulation, and signaling. J. Exp. Bot. 58:2339-2358 https://doi.org/10.1093/jxb/erm121
  11. Giuliano, G., R. Tavazza, G. Diretto, P. Beyer, and M. A. Taylor. 2008. Metabolic engineering of carotenoid biosynthesis in plants. Trends Biotechnol. 26:139-145 https://doi.org/10.1016/j.tibtech.2007.12.003
  12. Hancock, R.D. and R. Viola. 2005. Improving the nutritional value of crops through enhancement of L-ascorbic acid (vitamin C) content: rationale and biotechnological opportunities. J. Agric. Food Chem. 53:5248-5257 https://doi.org/10.1021/jf0503863
  13. Havaux, M., L. Dall'Osto, S. Cuine, G. Giuliano, and R. Bassi. 2004. The effect of zeaxanthin as the only xanthophylls on the structure and function of the photosynthetic apparatus in Arabidopsis thaliana. J. Biol. Chem. 279:13878-13888 https://doi.org/10.1074/jbc.M311154200
  14. Hounsome, N., B. Hounsome, D. Tomos, and G. Edwards-Jones. 2008. Plant metabolites and nutritional quality of vegetables. J. Food Sci. 73:R48-65 https://doi.org/10.1111/j.1750-3841.2008.00716.x
  15. Howitt, C.A. and B.J. Pogson. 2006. Carotenoid accumulation and function in seeds and non-green tissues. Plant Cell Environ. 29:435-445 https://doi.org/10.1111/j.1365-3040.2005.01492.x
  16. Ishikawa, T., J. Dowdle, and N. Smirnoff. 2006. Progress in manipulating ascorbic acid biosynthesis and accumulation in plants. Physiol. Plant. 126:343-355 https://doi.org/10.1111/j.1399-3054.2006.00640.x
  17. Jung, H.S., Y.T. Ko, and S.J. Lim. 1985. Effect of sugars on Kimchi fermentation and on the stability of ascorbic acid. Kor. J. Food. Nutr. 18:36-45
  18. Kim, H.J., W.K. Chang, M.K. Kim, S.S. Lee, and B.Y. Choi. 2002. Dietary factors and gastric cancer in Korea: a case-control study. Int. J. Cancer 97:531-535 https://doi.org/10.1002/ijc.10111
  19. Kim, J.-Y., E.-J. Lee, S.-K. Park, G.-W. Choi, and N.-K. Baek. 2000. Physicochemical quality characteristics of several Chinese cabbage (Brassica pekinensis RuPR) cultivars. Kor. J. Hort. Sci. & Technol. 18:348-352
  20. Linster, C.L. and S.G. Clarke. 2008. L-ascorbate biosynthesis in higher plants: the role of VTC2. Trends Plant Sci. 13:567-573 https://doi.org/10.1016/j.tplants.2008.08.005
  21. MacEvilly, C. and K. Peltola. 2003. The effect of agronomy storage processing and cooking on bioactive substances in food, p. 226-239. In: G. Goldberg (ed.). Plants: Diet and Health. Blackwell Publishing Ltd., Oxford, U.K
  22. Mares-Perlman, J.A., A.E. Millen, T.L. Ficek, and S E. Hankinson. 2002. Can lutein protect against chronic disease. J. Nutr. 132:518S-524S
  23. Orcutt, D.M. and E.T. Nilsen. 2000. The physiology of plants under stress soil and biotic factors. John Wiley and Sons Inc., New York
  24. Romer, S., P.D. Fraser, J.W. Kiano, C.A. Shipton, N. Misawa, W. Schunch, and P.B. Bramley. 2000. Elevation of the provitamin A content of transgenic tomato plants. Nat. Biotechnol. 18:666-669 https://doi.org/10.1038/76523
  25. Sauer, N. 2007. Molecular physiology of higher plant sucrose transporters. FEBS Lett. 581:2309-2317 https://doi.org/10.1016/j.febslet.2007.03.048
  26. Sujak, A., J. Gabrielska, and W. Grudzinski. 1999. Lutein and zeaxanthin as protectors of lipid membranes against oxidative damage: The structural aspects. Arc. Biochem. Biophys. 371:301-307 https://doi.org/10.1006/abbi.1999.1437
  27. USDA U.S., 2008. Search the USDA National Nutrient Database for Standard Reference, Release 21. Nutrient Data Laboratory Home Page, http://www.nal.usda.gov/fnic/foodcomp/search
  28. Vishnevetsky, M., M. Ovaids, and A. Vainstein. 1999. Carotenoid sequestration in plants: the role of carotenoid-associated proteins. Trends Plant Sci. 4:232-235 https://doi.org/10.1016/S1360-1385(99)01414-4
  29. WHO/FAO/UNU. 2007. Protein and amino acid requirements in human nutrition. WHO Press, p. 135-159
  30. Young, V.R. 1994. Adult amino acid requirements: the case for a major revision in current recommendations. J. Nutr. 124:1517S-1523S
  31. Young, V.R. and A.M. Ajami. 2000. Glutamate: An amino acid of particular distinction. J. Nutr. 130:892S-900S
  32. Yu, B., D.J. Lydiate, L.W. Young, U.A. Sch?er, and A. Hannoufa. 2008. Enhancing the carotenoid content of Brassica naus seeds by downregulating lycopene epsilon cyclase. Transgenic Res. 17:573-585 https://doi.org/10.1007/s11248-007-9131-x