Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
권호 표지

The High-Voltage Electron Microscopy in Biomedical Research

고압전자현미경의 의.생물학 연구 분야에서의 활용

Kim, Hyun-Wook;Kim, Jee-Woong;Rhyu, Im-Joo
김현욱;김지웅;유임주

  • Published : 20090600
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Abstract

Transmission electron microscopy (TEM) provides high resolution images, which are useful in studying ultrastructure of cells and tissues. We have to use very thin section about 60$\sim$100 nm thickness due to poor penetration power of the conventional TEM at 100 kV. To overcome this limitation, TEMs using higher accelerating voltage have been developed. TEMs can be categorized into conventional TEM, intermediate TEM, high voltage TEM (HVEM), and ultrahigh voltage TEM according to their accelerating voltage. HVEM using 500$\sim$1,000 kV has an enough penetration power to observe thick specimen up to 3$\sim$4 $\mu$m, which is useful understanding 3 dimensional configuration of the cell and tissue. HVEM was built up in Korea Basic Science Institute (KBSI, Daejeon, Korea) at 2004, maximum accelerating voltage is 1.3 MV in Korea. Many results showed up to the present various fields of science such as medical science, biology, agriculture and so on. Here, we briefly summarize recent biomedical applications of HVEM to provide an insight of HVEM for morphologist.

전자현미경(Electron Microscopy)은 우수한 해상력을 제공하여, 조직이나 세포의 미세구조 연구에 다양하게 이용되나, 전자빔 투과력의 한계로 100 nm 전후의 매우 얇은 조직만을 관찰할 수 있는 제한점을 갖는다. 따라서, 좀 더 두꺼운 조직을 관찰하기 위해서 높은 가속전압을 이용하는 현미경이 개발되었다. 전자현미경의 투과력은 사용하는 가속전압에 의해 증가되며, 전압의 크기에 따라 전자현미경(Conventional Electron Microscopy), 중전압현미경(Intermediate Voltage Electron Microscopy), 고압전자현미경(High Voltage Electron Microscopy: HVEM) 및 초고압전자현미경(Ultra High Voltage Electron Microscopy)의 네 가지로 구분된다. 이중 고압전자현미경은 500~1,000 kV의 높은 가속전압에 의해 증가된 투과력을 이용, 통상적인 전자현미경보다 두꺼운 시료를 관찰할 수 있다는 장점과 높은 해상력으로 조직이나 세포의 3차원적 구조 연구에 많이 이용되고 있다. 우리나라는 2004년 초고압전자현미경이 설치되어 운용 중이며, 의생물 분야뿐만 아니라 재료 기술분야에서도 활발하게 이용되고 있다. 이 논문에서는 고압전자현미경에 대한 간단한 설명과 최근 발표된 연구결과를 소개하여 형태학자들의 이해를 돕고자 한다.

Keywords

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