Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
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Transformation of Chrysanthemum morifolium with Insecticidal Gene (Cry1Ac) to Develop Pest Resistance

Soh, Ho-Seob;Han, Young-Hee;Lee, Gee-Young;Lim, Jae-Wook;Yi, Bu-Young;Lee, Youn-Hyung;Choi, Geun-Won;Park, Young-Doo

  • Published : 20090200
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Abstract

The objective of this study was to generate a pest-resistant Chrysanthemum morifolium by transforming crylAc gene, which has resistant to pests belonging to Noctuidae family that causes problems that damage to leaves and flowers during cultivation, into Chrysanthemum morifolium. For plant regeneration, standard type Jinba (JB) and Suhonochikara Yellow (SY) cultivars cultured on the medium supplemented with NAA 1.0 mg.L$^{-1}$ and BA 2.0 mg.L$^{-1}$ combination and spray type EuroYellow (EY) cultivar supplemented with NAA 1.0 mg.L$^{-1}$ and BA 1.0 mg.L$^{-1}$ combination showed the highest regeneration rates, respectively. When selectable reagents were added to selective medium for leaf segment culture, regeneration was inhibited on concentrations of above 0.5 mg.L$^{-1}$, 5.0 mg.L$^{-1}$ and 10.0 mg.L$^{-1}$ hygromycin for SY, EY and JB cultivars, respectively, and 0.5 mg.L$^{-1}$ phosphinothricin for all JB, SY and EY cultivars. When transgenic Chrysanthemum morifolium transformed by pGR-BT vector was analyzed by PCR, the expected size of 479 bp was observed on 29 JB lines, 13 SY lines and 5 EY lines. For pMJ-BT vector, 3 JB lines, 3 SY lines, and 1 EY line were selected as transformants. Finally, transformed lines selected by PCR analyses were inoculated with Spodoptera litura (tobacco cutworm) and showed that there was no larvae growth from 6 JB lines, 1 SY line and 1 EY line.

Keywords

References

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