Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Use of Nitrate-nitrogen as a Sole Dietary Nitrogen Source to Inhibit Ruminal Methanogenesis and to Improve Microbial Nitrogen Synthesis In vitro

  • Guo, W.S. (College of Animal Science and Technology, State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Schaefer, D.M. (Department of Animal Sciences, University of Wisconsin-Madison) ;
  • Guo, X.X. (College of Animal Science and Technology, State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Ren, L.P. (College of Animal Science and Technology, State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Meng, Qingxiang (College of Animal Science and Technology, State Key Laboratory of Animal Nutrition, China Agricultural University)
  • Received : 2008.06.30
  • Accepted : 2008.11.12
  • Published : 2009.04.01
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Abstract

An in vitro study was conducted to determine the effect of nitrate-nitrogen used as a sole dietary nitrogen source on ruminal fermentation characteristics and microbial nitrogen (MN) synthesis. Three treatment diets were formulated with different nitrogen sources to contain 13% CP and termed i) nitrate-N diet (NND), ii) urea-N diet (UND), used as negative control, and iii) tryptone-N diet (TND), used as positive control. The results of 24-h incubations showed that nitrate-N disappeared to background concentrations and was not detectable in microbial cells. The NND treatment decreased net $CH_4$ production, but also decreased net $CO_2$ production and increased net $H_2$ production. Total VFA concentration was lower (p<0.05) for NND than TND. Suppression of $CO_2$ production and total VFA concentration may be linked to increased concentration of $H_2$. The MN synthesis was greater (p<0.001) for NND than UND or TND (5.74 vs. 3.31 or 3.34 mg/40 ml, respectively). Nitrate addition diminished methane production as expected, but also increased MN synthesis.

Keywords

References

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