Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Pathological changes of the heart of olive flounder Paralichthys olivaceus in experimental Streptococcus parauberis infection

Streptococcus parauberis 인위 감염에 의한 넙치, Paralichthys olivaceus 심장의 병리학적 변화

  • Kim, Jin-Woo (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute) ;
  • Cho, Mi-Young (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute) ;
  • Won, Kyoung-Mi (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute) ;
  • Kim, Byoung-Gwan (Aquatic Animal Disease Control Center, National Fisheries Research and Development Institute) ;
  • Choi, Hee-Jung (Pathology Division, National Fisheries Research and Development Institute) ;
  • Han, Myoung-Chul (Seaworld Team, Hanwha 63 City) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute)
  • 김진우 (국립수산과학원 수산동물방역센터) ;
  • 조미영 (국립수산과학원 수산동물방역센터) ;
  • 원경미 (국립수산과학원 수산동물방역센터) ;
  • 김병관 (국립수산과학원 수산동물방역센터) ;
  • 최희정 (국립수산과학원 병리연구과) ;
  • 한명철 (한화 63시티) ;
  • 박명애 (국립수산과학원 병리연구과)
  • Published : 2009.12.01
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Abstract

We conducted bacteriological and histopathological analysis for olive flounder Paralichthys olivaceus after experimental infection with Streptococcus parauberis (FP2284) isolated from diseased olive flounder under different stress conditions. Experimental challenge was performed in healthy flounder (40.4 g in average body weight) by intraperitoneal (i.p.) injection with $2{\times}10^{8}$ CFU/fish under normal (no stress) or netting (for 2 min, twice/day) stress condition. The cumulative mortalities of no-stress and netting stress group were 70% and 95%, respectively. The most prevalent changes observed in experimentally infected flounder were darkness of skin and inflammation of the heart. Severe pericarditis, myocarditis and fibrosis were observed in the heart of the affected flounder. The results of viable counts showed the number of bacteria of the heart tissue was maintained over the $10^{4}$ CFU $g^{-1}$ heart for 3 weeks after inoculation. Histological lesions of the heart was more extensive and gradual decrease in bacterial numbers of heart tissue was delayed under stress condition.

병든 넙치에서 분리한 Streptococcus parauberis (FP2284)를 스트레스 조건을 달리하여 넙치, Paralichthys olivaceus에 인위감염한 후 조직 내 생균수 변화 및 병리조직학적 변화를 분석하였다. 인위감염 실험은 건강한 넙치 (평균 어체 중 40.4 g)에 $2{\times}10^{8}$ CFU/fish 농도로 복강주사하고 스트레스를 주지 않거나 하루 2번, 2분간 Netting 스트레스를 처리하였다. 스트레스를 주지 않은 그룹과 Netting 스트레스를 준 그룹의 누적 폐사율은 각각 70% 및 95%로 나타났다. 인위감염 시킨 넙치에서는 체색흑화와 심장의 염증이 주요 임상소견으로 나타났으며, 심장에서는 주로 심외막염, 심근염 및 심근의 섬유화가 관찰되었다. 인위감염 후 심장 조직의 세균수 변화를 측정한 결과, 주사 후 3주째까지 심장 1 g당 $10^{4}$ CFU의 세균수가 유지되는 것으로 나타났다. 스트레스 조건하에서 심장에서의 병리조직학적 변화가 더 심한 것으로 나타났으며, 심장 조직내 세균의 점차적인 감소가 지연되는 것으로 나타났다.

Keywords

References

  1. Caruso, D., Schlumberger, O., Dahm, C. and Proteau, J-P.: Plasma lysozyme levels in sheatfish Silurus glanis (L.) subjected to stress and experimental infection with Edwardsiella tarda. Aquaculture Res., 33:999-1008, 2002 https://doi.org/10.1046/j.1365-2109.2002.00716.x
  2. Collins, C.H. and Lyne, P.M.: Microbiological methods (4th edition). Butterworth, London, 521 pp., 1976
  3. Domenech, A., Ferenández-Garayzábal, J.F., Pascual, C., Garcia, J.A., Cutuli, M.T., Moreno, M.A., Collins, M.D. and Dominguez, L.: Streptococcosis in cultured turbot, Scophthalmus maximus (L.), associated with Streptococcus parauberis. J. Fish Dis., 19:33-38, 1996 https://doi.org/10.1111/j.1365-2761.1996.tb00117.x
  4. Eldar, A., Lawhon, S., Frelier, P.F., Assenta, L., Simpson, B.R., Varner, P.W. and Bercovier, H.: Restriction fragment length polymorphism of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel. FEMS Microbiol. Lett., 151:155-162, 1997 https://doi.org/10.1111/j.1574-6968.1997.tb12564.x
  5. Eldar, A. and Ghittino, C.: Latococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss: similar, but different diseases. Dis. Aquat. Org., 36: 227-231, 1999 https://doi.org/10.3354/dao036227
  6. Eldar, A., Goria, M., Ghittino, C., Zlotikin, A. and Bercovier, H.: Biodiversity of Lactococcus garvieae strains isolated fish in Europe, Asia, and Australia. Appl. Environ. Microbiol., 65:1005-1008, 1999
  7. Ghittino, C., Latini, M., Agnetti, F., Panzieri, C., Lauro, L., Ciappelloni, R. and Petracca, G.: Emerging pathologies in aquaculture: Effects on production and food safety. Vet. Res. Communi., 27:471-479, 2003 https://doi.org/10.1023/B:VERC.0000014204.37722.b6
  8. Kim, J.H., Gomez, D.K., Baeck, G.W., Shin, G.W., Heo, G.J., Jung, T.S. and Park, S.C.: Patho-genicity of Streptococcus parauberis to Olive Flounder Paralichthys olivaceus. Fish Pathol., 41(4):171-173, 2006 https://doi.org/10.3147/jsfp.41.171
  9. Kimura, H. and Kusuda, R.: Studies on the pathogenesis of streptococcal infection in cultured yellowtails Seriola spp.: effect of the cell free culture on experimental streptococcal infection. J. Fish Dis., 2:501-510, 1979 https://doi.org/10.1111/j.1365-2761.1979.tb00411.x
  10. Klesius, P.H., Shoemaker, C.A. and Evans, J.J.: Efficacy of single and combined Streptococcus iniae isolate vaccine administered by intraperitoneal and intramuscular routes in tilapia (Oreochromis niloticus). Aquaculture, 188:237-246, 2000 https://doi.org/10.1016/S0044-8486(00)00345-8
  11. Kusuda, R., Kawai, K., Salati, F., Banner, C.R. and Fryer, J.L.: Enterococcus seriolicida sp. nov., a fish pathogen. Int. J. System. Bacteriol., 41:406-409, 1991 https://doi.org/10.1099/00207713-41-3-406
  12. Mata, A.I., Gibello, A. Casamayor, Blanco, M.M., Domíngguz, L. and Frernández-Garayzabal, J.F.: Multiplex PCR assay for detection of bacterial pathogens associated with warm water streptococcosis in fish. Appl. Envir. Microbiol., 70:3183-3187, 2004 https://doi.org/10.1128/AEM.70.5.3183-3187.2004
  13. Nieto, J.M., Devesa, S., Quoroga, I. and Toranzo, A.E.: Pathology of Enterococcus sp. infection in farmed turbot, Scophthalmus maximus L. J. Fish Dis., 18:21-30, 1995 https://doi.org/10.1111/j.1365-2761.1995.tb01262.x
  14. Perera, R.P., Johnson, S.K., Collins, M.D. and Lewis, D.H.: Streptococcus iniae associated with mortality of Tilapia nilotica×T. aurea hybrids. J. Aquat. Anim. Health, 6:335-340, 1994 https://doi.org/10.1577/1548-8667(1994)006<0335:SIAWMO>2.3.CO;2
  15. Perera, R.A., Fiske, R.A. and Johnson, S.K.: Histopathology of Hybrid Tilapias Infected with a Biotype of Streptococcus iniae. J. Aquat. Anim. Health, 10:294-299, 1998 https://doi.org/10.1577/1548-8667(1998)010<0294:HOHTIW>2.0.CO;2
  16. Plumb, J.A.: Streptococcus and Enterococcus septicemia. In: Health maintenance of cultured fishes. Principal microbial diseases. CRC Press, Boca Raton, FL, 231-235, 1994
  17. Rasheed, V. and Plumb, J.A.: Pathogenicity of a non-haemolytic group B Streptococcus sp. in gulf killifish (Fundulus grandis Baird and Girard). Aquaculture, 37:97-105, 1984 https://doi.org/10.1016/0044-8486(84)90067-X
  18. Romalde, J.L., Magarinos, B., Villar, C., Barja, J.L. and Toranzo, A.E.: Genetic analysis of turbot pathogenic Streptococcus parauberis strains by ribotyping and random amplified polymorphic DNA. FEMS Microbiol. Lett., 459:297-304, 1999 https://doi.org/10.1111/j.1574-6968.1999.tb08741.x
  19. Toranzo, A.E., Magarinos, B. and Romalde, J.L.: A review of the main bacterial fish diseases in mariculture systems. Aquaculture, 246:37-61, 2005 https://doi.org/10.1016/j.aquaculture.2005.01.002
  20. Vendrell, D., Balcazar, J.L., Ruiz-Zarzuela, I., Blas, I., Girones, O. and Muzquiz, J.L.: Safety and efficacy of an inactivated vaccine against Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss). Rrev. Vet. Med., 80:222-229, 2007 https://doi.org/10.1016/j.prevetmed.2007.02.008
  21. 강철영, 강봉조, 문영건, 김기영, 허문수: 제주도 양식넙치 (Paralichthys olivaceus)로부터 분리한 비 용혈성 연쇄구균의 동정. 한국어병학회지, 20:109-117, 2007
  22. 김종훈, 김은희: 넙치 (Paralichthys olivaceus) 병어에서 분리된 연쇄상구균의 다양성. 한국수산학회지, 36(6):654-660, 2003
  23. 김현정, 우승호, 김진우, 박수일: Streptococcus iniae의 형태학적 특성과 병원성. 한국어병학회지, 18(2):167-178, 2005
  24. 우승호, 김현정, 이주석, 김진우, 박수일: 해수 양식어류에서 분리된 연쇄상구균의 종류와 병원성. 한국어병학회지, 19:17-33, 2006
  25. 이덕찬, 이재일, 박찬일, 박수일: 해산 양식어류로부터 분리된 연쇄구균증의 원인균, Lactococcus garvieae에 대한 연구. 한국어병학회지, 14:71-80, 2001
  26. 이창훈, 김필연, 고창식, 오덕철, 강봉조: 제주지역 양식 넙치 (Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적 특성. 한국어병학회지, 20:33-40, 2007
  27. 정용욱, 강철영, 김민주, 허문수, 오덕철, 강봉조:제주지역 양식넙치의 연쇄구균증 발생동향 및 원인균에 대한 분자적 동정. 한국미생물학회지, 42(3):199-204, 2006
  28. 조미영, 오윤경, 이덕찬, 김재훈, 박명애: 양식 넙치에서 분리한 Streptococcus parauberis의 동정방법에 따른 지역적 비교. 한국어병학회지, 20:49-60, 2007
  29. 조미영, 이재일, 김명석, 최희정, 이덕찬, 김진우:양식 강도다리, Platichthys stellatus Pallas에서 분리된 Streptococcus parauberis의 특성. 한국어병학회지, 21(3):209-217,2008
  30. 황성돈, 우승호, 김수현, 하수진, 정영은, 박수일:넙치, Paralichthys olivaceus 병어에서 분리된 Streptococcus parauberis의 면역 저항 특성. 한국어병학회지, 21(3):157-166, 2008