Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Characteristics of Wheat Germ Oil during Enzymatic Ethanolysis in Supercritical Carbon Dioxide

초임계 이산화탄소에서 밀배아유의 효소적 에탄올화 반응 특성

  • Back, Sung-Sin (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Kwon, Kyung-Tae (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Jung, Go-Woon (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Ahn, Hyaung-Min (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Sim, Jeong-Eun (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Kang, Hee-Moon (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University)
  • 백성신 (부경대학교 식품공학과) ;
  • 권경태 (부경대학교 식품공학과) ;
  • 정고운 (부경대학교 식품공학과) ;
  • 안향민 (부경대학교 식품공학과) ;
  • 심정은 (부경대학교 식품공학과) ;
  • 강희문 (부경대학교 식품공학과) ;
  • 전병수 (부경대학교 식품공학과)
  • Received : 2009.06.08
  • Accepted : 2009.08.18
  • Published : 2009.10.31
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Abstract

Enzymatic ethanolysis of wheat germ oil with immobilized lipase was investigated for enhancing the function of wheat germ oil. Ethanolysis reactions were carried out in two different systems; non-pressurized and pressurized system. In non-pressurized system, the enzymatic ethanolysis was carried out in an erlenmeyer flask(25 ml) containing a mixture of wheat germ oil and 99.90% ethanol using 1~5 wt% immobilized lipase as Lipozyme TL-IM and Lipozyme RM-IM and the reaction mixtures were incubated at $40{\sim}70^{\circ}C$ with 120 rpm shaking. In pressurized system, the enzymatic ethanolysis was carried out at various condition; immobilized lipase concentration(2 wt%), reaction time(24 h), reaction temperature($40{\sim}60^{\circ}C$) and reaction pressure(75, 100, 150, 200 bars). The samples obtained from each fraction were analyzed by HPLC for analysing contents of monoglyceride, diglyceride, and triglyceride. The conversion of wheat germ oil relied on the reaction temperature and the concentration of immobilized lipase. The optimum condition of enzymatic ethanolysis in non-pressurized and pressurized systems was at $50^{\circ}C$ and 100 bar.

이번 연구에서는 밀배아유의 기능성 향상을 위해 고정화 효소를 이용한 밀배아유의 효소적 에탄올 반응을 수행했고, 효소적 에탄올 반응의 비가압조건과 가압조건을 중점적으로 비교 분석했다. 비가압조건 효소적 에탄올 반응 수행은 밀배아유와 99.9% 에탄올 혼합물에 두 가지 고정화 효소인 Lipozyme TL-IM과 Lipozyme RM-IM를 1~5 w%(밀배아 기준 무게비)로 25 ml 플라스크에 shaking machine 상에서 $40{\sim}70^{\circ}C$, 120 rpm 조건으로 실험을 수행했다. 가압조건상에서의 효소적 에탄올 반응 조건은 고정화 효소 2 w%, 반응 시간 24시간, 반응 온도 $40{\sim}60^{\circ}C$ 및 반응 압력 75, 100, 150, 200 bar으로 수행했다. 실험으로부터 회수된 sample은 트리글리세라이드의 분해 정도를 살펴보기 위해 모노-, 디-, 트리글리세라이드를 HPLC를 이용하여 분석했다. 밀배아유의 전반적인 전환율은 반응온도와 고정화 효소의 농도에 따라 증가했고, 최적 반응 조건은 가압조건 $50^{\circ}C$, 100 bar이었다.

Keywords

Acknowledgement

Supported by : 한국산업기술재단, 중소기업청

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