Exercise Science (운동과학)

korean Society of Exercise Physiology (한국운동생리학회)
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Effects of diverse exercise Intensities on levels of MDA, SOD, GPx, and $TNF-\alpha$ in human plasma

다양한 운동강도가 혈장 지질과산화물, 항산화효소 및 염증성 사이토카인에 미치는 영향

  • Kim, Si-Yeong ;
  • Song, Uk
  • 김시영 (서울대학교) ;
  • 송욱 (서울대학교)
  • Published : 20090000
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Abstract

We investigated the effects of diverse exercise intensities on levels of MDA, SOD, GPx, and $TNF-\alpha$ in human plasma. Twelve volunteers participated in the exercise program which consisted of four intensities [40, 60, 80, and 100% of heart rate reserve(HRR)] on the treadmill and resting conditions. To determine whether levels of MDA, SOD, GPx, and $TNF-\alpha$ are dependent on exercise intensity, we used commercial assay kits, respectively using isolated human plasma, collected immediately after exercise. As exercise intensity increases, the levels of MDA(p<.05) and SOD(p<.05) decreased to 60% HRR and 80% HRR respectively, and then continuously increased to 100% HRR. In addition, GPx level increased in proportion to the exercise intensity (p<.05), whereas $TNF-\alpha$ level was not changed significantly. In conclusion, the exercise-induced changes of MDA, SOD, and GPx levels in human plasma appears to be attributed to the physiological adaptation to lower exercise intensity and increased oxidative stress as a consequence of higher oxygen consumption.

본 연구의 목적은 동일한 대상자의 다양한 운동강도에서 생성된 지질과산화물과 그 방어기전으로서 항산화효소 발현은 물론 산화반응으로 유도된 염증성 사이토카인 활성 정도를 알아보는데 있다. 20대 성인남성 12명을 대상으로 최대운동부하검사를 통해 안정시 심박수와 최대심박수를 구한 후, 100% heart rate reserve(HRR)는 물론 HRR의 40%, 60%, 80% 강도의 트레드밀 운동(약 400kcal 소모량)을 모든 연구대상자들이 실시하였다. 안정시를 포함한 4가지 강도의 운동을 실시한 직후, 채혈된 혈액을 분리하여 혈장 MDA, SOD, GPx, 그리고 $TNF-\alpha$의 활성도를 정량화가 가능한 assay kit을 통해 분석하였다. 운동강도가 높을수록 MDA (p<.05)와 SOD(p<.05)는 각각 60% HRR와 80% HRR까지 감소하였으며, 이후 100% HRR까지는 증가하는 양상을 보였다. GPx는 운동강도에 따라 지속적으로 증가하였으며, 특히 안정시와 40%HRR에 비해 100% HRR 운동강도에서 유의한 증가 (p<.05)를 보인 반면에 운동강도에 따른 $TNF-\alpha$의 활성에는 유의한 차이는 보이지 않았다. 결론적으로 MDA, SOD, 그리고 GPx의 활성 변화는 낮은 운동강도에 대한 생리적 적응과 고강도에서 늘어난 산소섭취로 인한 산화적 스트레스의 증가에 기인한 것으로 사료된다.

Keywords

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