Embryogenesis and Early Ontogenesis of a Marine Medaka, Oryzias dancena

바다 송사리 Oryzias dancena의 난발생 및 자치어의 형태 발달

  • Song, Ha Yeun (Department of Aquaculture, Pukyong National University) ;
  • Nam, Yoon Kwon (Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Dong Soo (Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
  • 송하연 (부경대학교 양식학과) ;
  • 남윤권 (부경대학교 해양수산형질전환생물연구소) ;
  • 방인철 (순천향대학교 해양생명공학과) ;
  • 김동수 (부경대학교 해양수산형질전환생물연구소)
  • Received : 2009.10.08
  • Accepted : 2009.11.24
  • Published : 2009.12.31

Abstract

The egg development and morphological changes of larvae, juveniles and adults of Oryzias dancena were observed. Fertilized eggs were incubated at $25{\pm}1^{\circ}C$; the process of embryonic development was observed by light microscopy and based on diagnostic features of the developing embryos. The average time to hatch was 11 days after fertilization. The hatched larvae averaged $4.40{\pm}0.24mm$ in total length (TL). The yolk sacs of the larvae were almost absorbed at 3 days after hatching and $4.55{\pm}0.23mm$ TL. At 21 days after hatching, the larvae were $7.23{\pm}0.73mm$ TL and had reached the juvenile stage. First ovulation was about 9 weeks after hatching and at $22.58{\pm}2.73mm$ TL.

Oryzias dancena의 난발생 과정과 자치어의 형태발달에 관하여 관찰하였다. 수온 $25{\pm}1^{\circ}C$에서 수정란은 수정 후 1시간이 경과하면 배반이 형성되었다가 30분의 간격으로 4세포기까지 난할이 이루어졌다. 이후로 발생의 속도가 느려져 수정 후, 7시간 45분이 경과된 후 포배기에 이르렀다. 수정 후 5시간 30분 후에 상실기에 도달하였으며, 포배기는 수정 후 7시간 45분 후에 이루어졌다. 수정 후 11시간 30분이 경과된 후 낭배기에 이르렀고, 수정 후 1일 6시간 후, 신경배를 형성하였다. 심장 박동이 시작되는 시기는 수정 후 2일 4시간째에 이루어졌으며, 수정 후 6일 12시간째 장기에 혈관이 형성되었다. 수정 후 11일째에 부화하였고, 부화 직후 자어는 전장이 $4.40{\pm}0.24mm$였으며, 부화 3일 후에 난황이 완전히 흡수되어 후기 자어기로 이행하였다. 부화 후 21일째에 각 지느러미 기조가 완전하게 형성되어 치어기로 이행하였으며, 이 때 전장은 $8.67{\pm}0.87mm$였다. 최초 산란이 이루어지는 시기는 부화 후 9주째로 전장은 $22.58{\pm}2.73mm$였다.

Keywords

Acknowledgement

Supported by : 국토해양부

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