Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Early Gonadogenesis and Sex Differentiation of a Marine Medaka, Oryzias dancena (Beloniformes; Teleostei)

해산 송사리 Oryzias dancena의 초기 생식소 형성 및 성분화

  • Song, Ha Yeun (Department of Aquaculture, Pukyong National University) ;
  • Nam, Yoon Kwon (Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Dong Soo (Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
  • 송하연 (부경대학교 양식학과) ;
  • 남윤권 (부경대학교 해양수산형질전환생물연구소) ;
  • 방인철 (순천향대학교 해양생명공학과) ;
  • 김동수 (부경대학교 해양수산형질전환생물연구소)
  • Received : 2009.08.10
  • Accepted : 2009.09.10
  • Published : 2009.09.30
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Abstract

Sex differentiation and gonad development were investigated in a marine medaka species, Oryzias dancena (Beloniformes; Teleostei). The average time to hatch was 11 days post-fertilization (dpf) at $25^{\circ}C$. Primordial germ cell (PGC) was first observed at 5 dpf and migrated to presumptive gonadal area between the gut and pronephric duct at 9 dpf. Male and female gonads were morphologically differentiated at 12 days post-hatching (dph). Early oocytes at perinucleolus stage as well as the formation of spermatid and efferent duct were observed at 28 dph. At 6 weeks of age, the ovary exhibited yolk granulation in many oocytes, while testis possessed a considerable number of spermatogonia and spermatids. The first ovulation was observed in 9-week-old females, and at the same age, males contained fully-matured spermatozoa. Data obtained in this study indicate that the gonad differentiation of O. dancena is the typical type of differentiated gonochorism.

Oryzias dancena를 해양생태계 위해성 평가를 위한 해양 생물모델로 연구하기 위한 기초적인 연구로 초기 성분화와 생식소 발달 과정을 조직학적으로 조사하였다. 원시생식세포가 처음 관찰된 시기는 수정 후 5일째이고, 수정 후 9일째에 장과 앞신장관 사이의 생식소 형성 부근으로 이동한 것이 관찰되었다. 부화 후 12일째의 생식소는 암 수로 분화가 이루어졌다. 부화 후 28일째 난소에서는 주변인기의 난모세포가 관찰되었으며, 28일째의 정소에서는 정소 소관의 수와 정원세포의 증가를 확인할 수 있었다. 산란은 부화 후 9주째에 이루어졌으며, 이 시기의 난소에서는 난황과립이 난 전체에 형성되는 성숙난과 배란 직후 성숙난이 빠져나간 흔적이 관찰되었다. 수컷 생식소에서 역시 많은 수의 정세포가 관찰되었으며 사정 직후 정자가 빠져나간 부분이 관찰되었다. 이상의 결과 본 종은 초기 성분화 과정에 자성 단계를 거치지 않고 정소와 난소로 분화하는 분화형 자웅이체에 속한다.

Keywords

Acknowledgement

Supported by : 국토해양부

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