Anatomy & Biological Anthropology (해부∙생물인류학)

Korean Association of Physical Anthropologists (대한체질인류학회)
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Inhibitory Effect of Bear Bile on Compound 48/80-induced Mast Cell Activation and IgE-mediated Vascular Permeability

Compound 48/80에 의한 비만세포 활성화와 IgE에 의한 혈관투과성 증가에 대한 웅담의 억제효과

Yan, Guang-Hai;Choi, Yun-Ho;Chai, Ok-Hee;Song, Chang-Ho
연광해;최윤호;채옥희;송창호

  • Published : 20090300
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Abstract

The bear bile has been used as a traditional drug medicine and has been known to have anti-tumor, anti-inflammatory and anti-oxidant effects. The purpose of this study is to investigate the inhibitory effect of bear bile on compound 48/80-induced mast cell activation in vitro and anti-dinitrophenyl (DNP) IgE-mediated vascular permeability in vivo. For this, the effects of bear bile on the degranulation, histamine release, calcium influx and the change of the intracellular cAMP levels of rat peritoneal mast cells (RPMCs) and the influences of the oral treatment of bear bile on IgE-mediated cutaneous vascular permeability were studied. the results were as follows; the compound 48/80-induced degranulation, histamine release and calcium influx of RPMCs were inhibited by pretreatment with bear bile, the cAMP levels of RPMCs were increased by pretreatment with bear bile, and bear bile inhibited anti-DNP IgE-mediated cutaneous vascular permeability. From the above results, it is suggested that bear bile contains some substances which inhibit anti-DNP IgE-mediated vascular permeability and mast cell activation. Bear bile potentially may serve as an effective therapeutic agent for allergic diseases.

비만세포는 알레르기 질환의 병태생리에 중요한 역할을 한다. 웅담이 compound 48/80에 의한 비만세포의 활성화와 IgE에 의한 혈관투과성 증가를 억제하는지 알아보고, 그 약리기전을 구명하기 위하여 이 연구를 실시하였다. 시험관 내 실험에서 웅담이 compound 48/80에 의한 비만세포의 탈과립과 히스타민 유리, 비만세포 속으로의 칼슘유입, 비만세포 내의 cAMP 수준에 미치는 영향을 관찰하였다. 또한 생체 실험에서 웅담을 입안으로 투여한 다음 IgE에 의한 피부 혈관투과성 증가에 미치는 영향을 관찰하였다. 웅담 전처리에 의하여 compound 48/80에 의한 흰쥐 복강 비만세포의 탈과립과 비만세포로부터의 히스타민 유리, 비만세포 내로의 칼슘유입이 억제되었다. 복강 비만세포 내 cAMP 수준도 웅담에 의해 증가되었다. 또한 IgE에 의한 피부 혈관투과성 증가가 웅담의 전처리로 억제되었다. 이상의 결과로 미루어, 웅담은 compound 48/80에 의한 비만세포 활성화와 IgE에 의한 혈관투과성 증가를 억제하는 항알레르기성 물질을 함유하고 있다고 생각된다. 웅담은 비만세포 활성화로 야기되는 알레르기 질환의 치료제로 이용할 수 있을 것으로 기대된다.

Keywords

References

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