Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Simple Genotyping Method Using Ampdirect$^{\circledR}$ Plus and FTA$^{\circledR}$ Technologies: Application to the Identification of Transgenic Animals and Their Routine Genetic Monitoring

Nakanishi, Satoshi;Kuramoto, Takashi;Serikawa, Tadao

  • Published : 20090000
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Abstract

A novel genotyping method, the Amp-FTA method, has been developed by combining the advantages of the Ampdirect$^{\circledR}$ Plus and FTA$^{\circledR}$ technologies. This method allowed the direct PCR amplification of DNA from unpurified blood immobilized on the FTA$^{\circledR}$ card. PCR templates were prepared only by punching out the discs from the FTA$^{\circledR}$ card, which could lead to a foolproof way of genotyping. The PCR products obtained could be subjected to downstream analyses such as restriction enzyme treatment and direct sequencing. The Amp-FTA method was applicable to buccal swabs collected by a simple technique minimizing distress to animals. Therefore, the Amp-FTA method is ideal for the genotyping and genetic monitoring of laboratory animals.

Keywords

References

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