Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Effect of verapamil on VEGF expression and apoptosis in early wound scarring of the rabbit ear

토끼 귀에 발생한 초기 창상 반흔에 베라파밀이 VEGF의 발현 및 세포자멸사에 미치는 영향

  • Bae, Tae Hui (Department of Plastic and Reconstructive Surgery, Pathology, College of Medicine, Chung-Ang University) ;
  • Kim, Woo Seob (Department of Plastic and Reconstructive Surgery, Pathology, College of Medicine, Chung-Ang University) ;
  • Kim, Han Koo (Department of Plastic and Reconstructive Surgery, Pathology, College of Medicine, Chung-Ang University) ;
  • Kim, Mi Kyoung (Department of Plastic and Reconstructive Surgery, Pathology, College of Medicine, Chung-Ang University)
  • 배태희 (중앙대학교 의과대학 성형외과학 교실, 병리학교실) ;
  • 김우섭 (중앙대학교 의과대학 성형외과학 교실, 병리학교실) ;
  • 김한구 (중앙대학교 의과대학 성형외과학 교실, 병리학교실) ;
  • 김미경 (중앙대학교 의과대학 성형외과학 교실, 병리학교실)
  • Published : 2009.01.15
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Abstract

Purpose: Excessive scarring in the forms of keloid and hypertrophic scar could be a consquence of the accumulation of granulation tissue cells due to aberrant control of apoptosis. Verapamil retard extracelluar matrix production and inhibits VEGF production in primary cultured keloid fibroblast. The object of this study was effect of verapamil on VEGF expression and apoptosis in early wound scarring of the rabbit ear. Methods: Full thickness wounds were created on the ventral side of 6 New Zealand rabbits's ear. 16 days after initial wounding verapamil and saline were injected each scars and scars were harvested 1 week, 2 weeks, 4 weeks later. The wounds were stained with hematoxylin and eosin, TUNEL stain, immunohistochemical stain for VEGF and calculated scar elevation index. Results: Histologic analaysis demonstrated significant reduction in inflammation, vascularity and improvement in dermal collagen organization in experimental group. In TUNEL staining apotosis positive cells were increased and immunohistochemial staining of VEGF demonstrated significant reduction of VEGF expression in experimental group. No significant difference was noted in scar elevation index between two groups. Conclusion: This study suggest that intralesional injection of verapamil on early wound scarring of the rabbit ear decreased VEGF production and increased apoptosis and have a benefit on the pathophysiology of scar formation.

Keywords

References

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