Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Relationship between Populations and Analysis of Genetic Structure in the Korean Native Chicken and the Endemic Chicken Breeds

한국재래닭 및 토착화 품종간의 유연 관계 및 유전 특성 분석

  • Oh, J.D. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Kang, B.S. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Kim, H.K. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Park, M.N. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Chae, E.J. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Seo, O.S. (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Genomic Informatics Center, Hankyong National University)
  • 오재돈 (농촌진흥청 축산과학원 가금과) ;
  • 강보석 (농촌진흥청 축산과학원 가금과) ;
  • 김학규 (농촌진흥청 축산과학원 가금과) ;
  • 박미나 (농촌진흥청 축산과학원 가금과) ;
  • 채은진 (농촌진흥청 축산과학원 가금과) ;
  • 서옥석 (농촌진흥청 축산과학원 가금과) ;
  • 이학교 (한경대학교 유전정보연구소) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소)
  • Published : 2009.01.02
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to assess the genetic variation and establish the relationship amongst breeds and strains using 7 chicken specific microsatellite markers. A total of 317 DNA samples from four Korean native chicken (KNC) strains (KR: Korean Native Red chicken strain, KY: Korean Native Yellow chicken strain, KL: Korean Native Black chicken strain, KO: Ogol chicken strain) and three introduced endemic chicken breeds (LE: Leghorn chicken breed, RI: Rhode Island Red chicken breed, CO: Cornish chicken breed). The size of microsatellite markers was decided using GeneMapper Software (v.4.0) after being analyzed using an ABI 3130 Genetic Analyzer. Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. The lowest distance (0.074) was observed between the KY and KL breeds and the highest distance (0.779) between the KL and LE breeds. The KNC strains (KR, KY, KL) have comparatively near genetic distance each other. On the other side, each individual was not ramified to different groups and were spread evenly in phylogenetic dendrogram about all the KNC of each strain populations. But the endemic breed populations (LE, RI, CO) were ramified to different groups. The microsatellite polymorphism data were shown to be useful for assessing the genetic relationship between Korean native strains and other foreign breeds.

본 연구는 한국재래닭의 유전적 특성과 차별성을 검증하기 위하여 microsatellite(MS) marker를 이용한 타품종들과의 유전적 유연 관계를 분석하였다. 분석을 위해 7개 계통의 닭 317수(백색레그혼: 79, 로드아일랜드레드: 40, 코니쉬: 37, 적갈재래닭: 44, 황갈재래닭: 39, 흑색재래닭: 39, 오골계: 39)를 대상으로 7개의 MS marker들을 이용해 대립 유전자 및 유전자형을 분석하였다. 7개의 집단간의 유전적 유연 관계를 알아보기 위해 각 MS marker별 대립 유전자의 빈도를 산출하여 이를 근거로 집단간의 유전적 거리에 대한 추정 결과 KY와 KL간의 유전적 거리는 0.074로 가장 가까운 것으로 나타났으며, KR과 KY(0.101), KR과 KL(0.173) 역시 가까운 유전적 거리를 나타내고 있음을 확인하였다. 로드아일랜드의 경우, 한국재래닭 3계통과의 유전적 거리가 평균 0.233으로 타품종에 비해 비교적 가까운 것으로 나타났다. 레그혼은 다른 모든 품종들과의 거리가 가장 먼 것으로 확인되었다. 또한, 산란종인 백색레그혼과 육용종인 코니쉬 간의 유전적 거리는 가장 먼 것으로 확인되었다. 분석된 집단간의 유전적 구조에 따라 각 개체들이 어떻게 분포되어 있는가를 확인하기 위하여 각 개체들간의 유전적 거리를 분석하였다. 분석 결과, 백색레그혼의 경우 하나의 큰 그룹으로 분포하고 있음을 확인하였다. 또한, 로드아일랜드레드, 코니쉬 및 오골계 역시 각각 그룹을 형성하여 분포하고 있음을 확인하였다. 한국 재래닭 3계통은 각각 그룹을 이루지 못하였으며, 3계통이 합쳐져 넓게 분포하고 있음을 확인하였다. 재래닭의 경우 넓게 분포되어 있기는 하지만 다른 품종들과의 분포의 차이가 있음을 확인할 수 있었다.

Keywords

References

  1. Arranz, JJ, Bayon Y, San Primitivo F 1996 Comparison of protein markers and microsatellites in differentiation of cattle populations. Anim Genet 27: 415-419. https://doi.org/10.1111/j.1365-2052.1996.tb00508.x
  2. Barker JSF, Tan SG, Selvaraj OS, Mukherjee TK 1997 Genetic variation within and relationships among populations of Asian water buffalo (Bubalus bubalis). Anim Genet 28:1-13. https://doi.org/10.1111/j.1365-2052.1997.00036.x
  3. Bjornstad G, Nilsen NO, Roed KH 2003 Genetic relationship between Mongolian and Norwegian horses? Anim Genet 34:55-58. https://doi.org/10.1046/j.1365-2052.2003.00922.x
  4. Blott SC, Williams JL, Haley CS 1999 Discriminating among cattle breeds using genetic markers. Heredity 82:613-619. https://doi.org/10.1046/j.1365-2540.1999.00521.x
  5. Botstein D, White RL, Skolnick M, Davis RW 1980 Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314- 331.
  6. Lee C, Pollak EJ 2002 Genetic antagonism between body weight and milk production in beef cattle. J Anim Sci 80: 316-321. https://doi.org/10.2527/2002.802316x
  7. Li K, Chen Y, Moran C, Fan B, Zhao S, Peng Z 2000 Analysis of diversity and genetic relationships between four Chinese indigenous pig breeds and one Australian commercial pig breed. Anim Genet 31:322-325. https://doi.org/10.1046/j.1365-2052.2000.00649.x
  8. Mannen H, Tsuji S, Mukai F, Goto N, Ohtagaki S 1993 Genetic similarity using DNA fingerprinting in cattle to determine relationship coefficient. J Hered May-Jun;84(3):166-9.
  9. Martin-Burriel I, Garcia-Muro E, Zaragoza P 1999 Genetic diversity analysis of six Spanish native cattle breeds using microsatellites. Anim Genet 30:177-182. https://doi.org/10.1046/j.1365-2052.1999.00437.x
  10. Nei M, Taima F, Tateno Y 1983 Accuracy of estimated phylogenetic trees from molecular data. J Mol Evol 19:153-170. https://doi.org/10.1007/BF02300753
  11. Ota T 1993 DISPAN. Pennsylvania State University, PA. USA.
  12. Park DDE 2000 Microsatellite Toolkit for MS Excel 97 or 2000. (in personnel communication).
  13. Peelman LJ, Mortiaux F, Van Zeveren A, Dansercoer A, Mommens G, Coopman F, Bouquet Y, Burny A, Renaville R, Portetelle D 1998 Evaluation of the genetic variability of 23 bovine microsatellite markers in four Belgian cattle breeds. Anim Genet Jun;29(3):161-7. https://doi.org/10.1111/j.1365-2052.1998.00280.x
  14. Roy MS, Geffen E, Smith D, Ostrander EA, Wayne RK 1994 Patterns of differentiation and hybridization in North American wolflike canids, revealed by analysis of microsatellite loci. Mol Biol Evol 11(4):553-70.
  15. Saitou N, Nei M 1987 The neighborjoining method: A new method for reconstruction phylogenetic tree. Mol Biol 4: 406-425.
  16. Sneath PHA, Sokal RR 1973 Numerical Taxonomy. Freeman, San Francisco.

Cited by

  1. Single Nucleotide Polymorphisms (SNPs) Discovery in GHSR Gene and Their Association Analysis with Economic Traits in Korean Native Chickens vol.43, pp.4, 2016, https://doi.org/10.5536/KJPS.2016.43.4.273
  2. Identification of SNP(Single Nucleotide Polymorphism) from MC1R, MITF and TYRP1 associated with Feather Color in Chicken vol.41, pp.1, 2014, https://doi.org/10.5536/KJPS.2014.41.1.29
  3. DNA Markers for the Genetic Diversity in Korean Native Chicken Breeds: A Review vol.43, pp.2, 2016, https://doi.org/10.5536/KJPS.2016.43.2.63
  4. Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers vol.45, pp.3, 2018, https://doi.org/10.5536/KJPS.2018.45.3.229