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Transmission of Fusarium oxysporum by the Fungus Gnat, Bradysia difformis (Diptera: Sciaridae)

작은뿌리파리에 의한 Fusarium oxysporum의 전반

  • Kim, Hyeong-Hwan (Horticultural Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Jeon, Heung-Yong (Horticultural Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yang, Chang-Yeol (Horticultural Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kang, Taek-Joon (Horticultural Environment Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Han, You-Kyoung (Horticultural Environment Division, National Institute of Horticultural & Herbal Science, RDA)
  • 김형환 (농촌진흥청 원예특작과학원 원예특작환경과) ;
  • 전흥용 (농촌진흥청 원예특작과학원 원예특작환경과) ;
  • 양창열 (농촌진흥청 원예특작과학원 원예특작환경과) ;
  • 강택준 (농촌진흥청 원예특작과학원 원예특작환경과) ;
  • 한유경 (농촌진흥청 원예특작과학원 원예특작환경과)
  • Published : 2009.12.01

Abstract

The fungus gnat, Bradysia difformis was examined for its ability to transmit Fusarium oxysporum in PDA culture. Larvae and adults of B. difformis were able to transmit the fungus as ingested and sticking. We constructed GFP-expressed mutants with Fusarium oxysporum, then feed it to larvae of fungus gnat, B. difformis. So that mycelia were placed in the alimentary canal of larva.

PDA 배지상에서 작은뿌리파리 유충과 성충은 토양 전염성 병원균인 Fusarium oxysporum을 전반하였으며, 성충에 비해 유충의 전반이 높았다. 작은뿌리파리 유충은 F. oxysporum 균을 섭식하여 전반하였으며, 성충은 몸체에 균을 묻혀 전반하였다. 형광발현 형질전환 F. oxysporum 균을 제작하여 작은뿌리파리 유충에 먹인 결과 균은 소화관을 따라 분포하였다.

Keywords

References

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