Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A High-Yielding, Generic Fed-Batch Process for Recombinant Antibody Production of GS-Engineered Cell Lines

  • Fan, Li (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Zhao, Liang (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Sun, Yating (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Kou, Tianci (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Zhou, Yan (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Tan, Wen-Song (The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
  • Published : 2009.12.31
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Abstract

An animal-component-free and chemically defined fed-batch process for GS-engineered cell lines producing recombinant antibodies has been developed. The fed-batch process relied on supplying sufficient nutrients to match their consumption, simultaneously minimizing the accumulation of by-products (lactate and osmolality). The proportionalities of nutritional consumption were determined by direct analysis. The robust, metabolically responsive feeding strategy was based on the offline measurement of glucose. The fed-batch process was shown to perform equivalently in GS-CHO and GS-NS0 cultures. Compared with batch cultures, the fed-batch technology generated the greater increase in cell yields (5-fold) and final antibody concentrations (4-8-fold). The majority of the increase in final antibody concentration was a function of the increased cell density and the prolonged culture time. This generic and high-yielding fed-batch process would shorten development time, and ensure process stability, thereby facilitating the manufacture of therapeutic antibodies by GS-engineered cell lines.

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References

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