Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Curcumin and Vit. E Alleviate Alone or Synergetically Hydrogen Peroxide Induced-Oxidative Stress on Boar Sperm Characteristics during In Vitro Storage

  • Jang, Hyun-Young (College of Animal Life Science, Kangwon National University) ;
  • Jin, Hyun-A (College of Animal Life Science, Kangwon National University) ;
  • Lee, Hee-Young (College of Animal Life Science, Kangwon National University) ;
  • Kim, Dae-Jung (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Jong-Taek (School of Veterinary Medicine, Kangwon National University) ;
  • Park, In-Chul (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University)
  • Published : 2009.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Antioxidants partially ameliorated the detrimental effects of reactive oxygen species (ROS) on sperm characteristics during in vitro storage. The objective of the present study was to investigate the single or synergetic antioxidative effect of curcumin and Vit. E on the characteristics of fresh boar sperm during in vitro storage. The sperm viability in curcumin, Vit. E supplementation and curcumin+Vit. $E+H_2O_2$ groups remained over 85.0% in 3 hr incubation period, but in 6 hr incubation period, curcumin+Vit. $E+H_2O_2$ groups was sharply dropped than those of curcumin and Vit. E group. The membrane intergrity in all evaluated groups except for $H_2O_2$ group did not significantly difference in 3 hr incubation period. The viability in curcumin or Vit. E supplementation were significantly increased than in curcumin+$H_2O_2$ and Vit. $E+H_2O_2$ group in 6 hr incubation period. The percentage of mitochondrial activity and acrosome intergrity obtained similar trends within same incubation periods irrespective of treatment. The lipid peroxidation of spermatozoal plasma membrane ranged from $11.6{\sim}17.5\;nM/l{\times}10^6$ and $14.0{\sim}19.0\;nM/l{\times}10^6$ in 3 hr and 6 hr incubation periods. In conclusion, curcumin or Vit. E surpplementation alone or cooperatively improved sperm viability index (motility, membrane intergrity, viability and survival rates) and fertility index (mitochondria activity, acrosome intergrity and lipid peroxidation) of fresh boar sperm, indicating that curcumin and Vit. E have a antioxidative properties through its scavenging activity against hydrogen peroxide.

Keywords

References

  1. Agarwal A, Ramadan A, Mohamed AB (2003): Role of reactive oxygen species in the pathophysiology of human reproduction. Fertile Steril 79:829-843 https://doi.org/10.1016/S0015-0282(02)04948-8
  2. Breininger E, Beorlegui NB, O'Flaherty, Beconi MT (2005): Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. 63:2126-2135
  3. Cai Q, Rahn RO, Zhang R (1997): Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Letters 119:99-107 https://doi.org/10.1016/S0304-3835(97)00261-9
  4. Chen CS, Chao HT, Pan RL, Wei YH (1997): Hydroxyl radical-induced decline in motility and increase in lipid peroxidation and DNA modification in human sperm. Biochem Mol Biol Inter 43:291-303 https://doi.org/10.1080/15216549700204071
  5. Cerolini S, Maldjian A, Surai P, Noble R (2000): Viability susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim Reprod Sci 58:99-111 https://doi.org/10.1016/S0378-4320(99)00035-4
  6. de Lamirande, O' Flaherty (2008): Sperm activation: role of reactive oxygen species and kinases. Biochimica et Biophysica Acta 1784:106-115 https://doi.org/10.1016/j.bbapap.2007.08.024
  7. Duvoix A, Blasius R, Delhalle S, Schnekenburger M, Morceau F, Henry E, Dicato M, Diederish M (2005): Chemopreventive and therapeutic effects of curcumin. Cancer Lett 223:181-190 https://doi.org/10.1016/j.canlet.2004.09.041
  8. Eybl V, Kotyzova D, Koutensky J (2006): Comparative study of natural antioxidants - curcumin, resveratrol and melatonin - in cadmium-induced oxidative damagein mice. Toxicology 225:150-156 https://doi.org/10.1016/j.tox.2006.05.011
  9. Gro$\beta$feld R, Sief B, Struckmann C, Frenzel A, Maxwell WM, Bath D (2008): New aspects of boar semen freezing strategies. Grossfeld 70:1225-1233
  10. Guthrie HD, Welch GR, Long JA (2008): Mitochondrial function and reactive oxygen species action in relation to boar motility. Theriogenology 70:1029- 1215
  11. Jackson JK, Higo T, Hunter WL, Burt HM (2006): The antioxidants curcumin and quercerthin inhibit inflammatory processes associated with arthritis. Inflamm Res 55:168-175 https://doi.org/10.1007/s00011-006-0067-z
  12. Johnson MK, Loo G (2000): Effects of epigallocatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat Res 28:211-218 https://doi.org/10.1016/0027-5107(75)90098-6
  13. Kumaresan A, Kadrivel G, Bujarbaruah KM, Bardoloi RK, Das A, Kumar S, Naskar S (2009): Presevation of boar semen at 18℃ induces lipid peroxidation and apoptosis like changes in spermatozoa. Anim Reprod Sci 110:162-171 https://doi.org/10.1016/j.anireprosci.2008.01.006
  14. Mathuria N, Verma RJ (2008): Curcumin ameliorates aflatoxin-induced toxicity in mice spermatozoa. Fert Steril 90:776-780
  15. Pena FJ, Johannisson A, Wallgren M, Rodriguez Marinez H (2003): Assenssment of fresh and frozen thawed boar semen using an annexin assay: a new method of evaluating sperm membrane intergrity. Theriogenology 60:277-289
  16. Sahoo DK, Roy A, Bhanja S, Chainy GBN (2008): Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation. Gen Comp Endocrinol 156:63-70 https://doi.org/10.1016/j.ygcen.2007.11.007
  17. Saleh RA, Agarwal A (2002): Oxidative stress and male infertility: From research bench to clinical practice. 23:737-752
  18. Sikka S (2004): Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl 25:5-18 https://doi.org/10.1002/j.1939-4640.2004.tb02751.x
  19. Subudhi U, Das K, Patial B, Bhanja S, Chainy GNB (2008): Alleviation of enhanced oxidative stress and oxygen consumption of L-thyroxine induced hyperthyroid rat liver mitochondria by vitamin E and curcumin. Chemico Biological Interactions 173:105- 114 https://doi.org/10.1016/j.cbi.2008.02.005
  20. Tavilani H, Goodarzi MT, Vaisi-Raygani A, Salimis, Hassanzadeh T (2008): Activity of antioxidant enzymes in seminal plasma and their relationship with lipid peroxidation of spermatozoa. Int Braz J Urol.
  21. Thuwanut P, Chatdarong K, Techakumphu M, Anxer E (2008): The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology 70:233-240 https://doi.org/10.1016/j.theriogenology.2008.04.005