Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Genotype Analysis of the Major Histocompatibility Complex Region in Korean Native Chicken

한국 재래닭의 MHC 영역 유전자형 분석

  • Jung, Kie-Chul (Poultry Science Division, National Institute of Animal Science, RDA) ;
  • Hoque, Md. Rashedul (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Seo, Dong-Won (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Park, Byung-Kwon (Department of Companion & Laboratory Animal Science, Kongju National University) ;
  • Choi, Kang-Duk (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Lee, Jun-Heon (Department of Animal Science and Biotechnology, Chungnam National University)
  • 정기철 (농촌진흥청 국립축산과학원 가금과) ;
  • 라세둘 (충남대학교 동물자원과학부) ;
  • 서동원 (충남대학교 동물자원과학부) ;
  • 박병권 (공주대학교 특수동물학과) ;
  • 최강덕 (한경대학교 생물정보통신대학원) ;
  • 이준헌 (충남대학교 동물자원과학부)
  • Published : 2009.12.31
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Abstract

The chicken major histocompatibility complex (MHC) is known to be associated with disease resistance and susceptibility to several pathogens. The microsatellite marker LEI0258 is physically located between the BG and BF of MHC region and variations near this marker have been well documented. In this report, the LEI0258 marker was used to find specific alleles for the Korean native chicken. The MHC haplotype was analyzed by PCR screening and sequencing of LEI0258 region in four different breeds including black Korean native chicken, brown Korean native chicken, Cornish and Rhode island red. The serologically same MHC haplotypes showed the differences in repeat numbers, a few indels or single nucleotide polymorphisms by sequencing analysis. Even though we could not identify specific alleles for Korean native chickens, the genotypes analyzed in these breeds can give valuable information for the relationships with disease resistance and establishment of breeding strategies for the Korean native chicken.

닭의 MHC 유전자 내에 있는 microsatellite marker LEI0258은 혈청의 구분 및 질병 저항성 유전자로 많은 연구가 되어 있다. 본 연구는 유전변이가 있는 microsatellite marker LEI 0258을 한국 재래계 흑색종, 한국 재래계 갈색종, 코니쉬종, 로드아일랜드 레드종에 적용하여 품종 특이 유전자형 및 allele을 탐색하고, 품종 구분 활용에 가능하지 여부를 실험하기 위하여 실시하였다. 한국 재래계만이 가지고 있는 특이 대립 유전자는 찾을 수는 없었지만 품종간 유전자형의 빈도 차이를 보이는 대립 유전자들을 확인할 수 있어 마커의 조합을 통하여 품종을 구분할 수 있는 가능성을 제시하였으며, 질병 저항성 연구의 기초 자료로 그 이용성이 높을 것으로 사료된다.

Keywords

References

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  1. DNA Markers for the Genetic Diversity in Korean Native Chicken Breeds: A Review vol.43, pp.2, 2016, https://doi.org/10.5536/KJPS.2016.43.2.63
  2. The breeding history and commercial development of the Korean native chicken vol.73, pp.01, 2017, https://doi.org/10.1017/S004393391600088X