Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Phylogeny Study of Genus Pelvetia in Korea by Internal Transcribed Spacer Sequence (ITS)

ITS에 의한 한국 내 Pelvetia속 분류군의 계통학적 연구

  • Lee, Bok-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Huh, Man-Kyu (Department of Molecular Biology, Dongeui University) ;
  • Choi, Joo-Soo (Department of Molecular Biology, Dongeui University) ;
  • Cho, Sung-Hyun (Department of Biology, Pennsylvania State University)
  • 이복규 (동의대학교 분자생물학과) ;
  • 허만규 (동의대학교 분자생물학과) ;
  • 최주수 (동의대학교 분자생물학과) ;
  • 조성현 (펜실베이니아 주립대학 생물학과)
  • Published : 2009.03.31
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Abstract

The brown algae, or phaeophytes, are a large group of multicellular algae, including many notable types of seaweed. We analysed intra- and interspecific phylogenic studies within the genus Pelvetia in Korea and compared them with results of both same and different species in GenBank. The sequences for P. babingtonii in Korea were generally similar to those for P. babingtonii AF102957, and the sequences of P. siliquosa in Korea were also similar to those of P. siliquosa AF102958. Sequence variation within the Pelvetia is mostly due to nucleotide substitutions, although several small indels and some large indels can be found. Another source of sequence divergence is length variation due to stretches of short repeats that occur at the ITS1 or ITS2 in all the Pelvetia. NJ analysis consists mainly of two clades. One of them contains P. canaliculata and P. limitata, and is sister to the rest of the genus (P. siliquosa, P. compressa, and P. babingtonii). P. babingtonii is not grouped one clade. In the MP analysis, ten accessions or populations were fully resolved and all accessions from the same species formed with 99% or 100% bootstrap supports.

갈조류 모자반과 Pelvetia속은 다세포 조류로 많은 해조류를 포함하고 있으며 북반구 태평양과 대서양에 분포되어 있다. Pelvetia속에 속하는 우리나라의 동종 및 근연한 같은 속내 종에 대해 유전자은행을 통해 밝혀진 ITS에 의한 서열을 이용하여 계통관계를 조사하였다. 한국의 P. babingtonii은 북미의 P. babingtonii AF102957과 유사한 핵산서열로 계통도 분석에서 같은 분지군을 형성하였다. 한국의 P. siliquosa은 역시 북미의 P. siliquosa AF102958과 유사한 핵산서열로 계통도 분석에서 같은 분지군을 형성하였다. Pelvetia 속 내 여러 종간은 결실이나 삽입에 의한 indel이 많은 반면 같은 종내 계통은 치환에 의한 차이가 현저하였다. 이 속은 NJ분석에서 크게 두 분지군으로 나뉘는데 한 그룹은 P. canaliculata와 P. limitata이며 나머지 그룹은 P. siliquosa, P. compressa, P. babingtonii을 포함하고 있었다. ITS 서열로 한국 내 분류군과 북미 간 분류군이 잘 구분되었다. MP 분석에서도 높은 지지도로 잘 구분되었다. 따라서 ITS 서열로 종 동정에 이용할 수 있었으며, 종의 보전이나 생식질 보전에 기초로 이용될 수 있을 것으로 사료된다.

Keywords

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