Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Radical Scavenging Activities and Antioxidant Constituents of Oriental Melon Extract

참외 추출물의 라디칼소거활성과 항산화 성분

  • Kim, Hye-Suk (Department of Horticulture, Kyungpook National University) ;
  • Hong, Mi-Jeong (Division of Applied Biosciences, Kyungpook National University) ;
  • Kang, In-Yeong (Department of Horticulture, Kyungpook National University) ;
  • Jung, Ji-Youn (Department of Horticulture, Kyungpook National University) ;
  • Kim, Hye-Kyung (Department of Horticulture, Kyungpook National University) ;
  • Shin, Yong-Seub (Seongju Fruit Vegetable Experiment Station, Gyeongbuk AR&ES) ;
  • Jun, Ha-Joon (Department of Horticulture, Daegu University) ;
  • Suh, Jun-Kyu (Department of Horticulture, Kyungpook National University) ;
  • Kang, Young-Hwa (Department of Horticulture, Kyungpook National University)
  • Published : 2009.12.31
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Abstract

Extracts from various parts of oriental melon were obtained and antioxidant property and antioxidant constituents including total phenol, total flavonoid, total vitamin C were examined. Free radical scavenging activity was measured by DPPH and ABTS method. Peel part of oriental melon showed the most potent scavenging activities against DPPH and ABTS radicals. The contents of total phenol, total flavonoid in peel were higher than other parts except vitamin C. The amount of vitamin C was the highest in placenta. The relationship between antioxidant activities and antioxidant constituents was determined and showed higher correlation coefficients between antioxidant activities and content of total phenol than other constituents. The above results suggest that phenolic compounds affect antioxidant activity of oriental melon and oriental melon has a good promise as functional food for enhancing health.

참외의 기능성을 규명하기 위해 참외의 부위별 추출물의 라디칼 소거활성 및 항산화 물질 함량을 측정하였다. 참외의 DPPH 라디칼 소거능은 2mg/mL 농도에서 껍질 부위가 74.7%로 가장 높게 나타났고, ABTS 라디칼 소거능에서도 DPPH 라디칼 소거능과 마찬가지로 껍질 부위에서 가장 높은 활성을 보였다. 총 페놀과 총 플라보노이드의 함량은 껍질 부위에서 생체 중 100g 당 각각 134.5mg, 159.1mg으로 가장 높은 함량을 나타냈고, 다음은 태좌, 과육 순이었다. 비타민 C 함량의 경우 총 페놀과 총 플라보노이드의 함량과는 다르게 태좌 부위에서 40.7mg으로 가장 높게 나타났다. 라디칼 소거활성과 항산화 물질 간의 상관관계를 조사해 본 결과 총 페놀과 총 플라보노이드의 함량과 항산화 활성 간에 고도의 상관관계가 나타났고, 비타민 C의 함량과는 다소 낮은 상관관계를 보였다. 이러한 결과들을 종합하면 참외껍질 부위에서 라디칼 소거활성과 항산화 성분함량이 가장 높게 나타났고, 참외의 항산화 활성을 나타내는 주요성분은 페놀성물질일 것으로 생각된다. 향후 라디칼 소거활성과 항산화 성분 함량에서 가장 높게 나타난 껍질 부위의 활성성분 규명 등에 대한 지속적인 연구가 필요할 것이다.

Keywords

References

  1. Ahn, M.S., M.S. Seo, and H.J. Kim. 2007. A study on the antioxidative and antimicrobial activities of the Citrus unshju peel extracts. Kor. J. Food Culture. 22(4):454-461
  2. Ames, B.N., M.K. Shigenaga, and T.M. Hagen. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. USA 90:7915-7922 https://doi.org/10.1073/pnas.90.17.7915
  3. Block, G., B. Patterson, and A. Subar. 1992. Fruit, vegetables, and cancer prevention: A review of the epidemiological evidence. Nutr. Cancer. 18:1-29 https://doi.org/10.1080/01635589209514201
  4. Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1202 https://doi.org/10.1038/1811199a0
  5. Branen, A.L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52(2):59-63 https://doi.org/10.1007/BF02901825
  6. Chiu, J.F., A.T Lau, and Y. Wang. 2008. Reactive oxygen species: current knowledge and applications in cancer research and therapeutic. J. Cell Biochem. 104(2):657-667 https://doi.org/10.1002/jcb.21655
  7. Cho, S.Y., Y.B. Han, and K.H. Shin. 2001. Screening for antioxidant activity of edible plants. J. Korean Soc. Food Sci. Nutr. 30(1):133-137
  8. Choi, S.Y., S.H. Lim, J.S. Kim, T.Y. Ha, S.R. Kim, K.S. Kang, and I.K. Hwang. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Kor. J. Food Sci. Technol. 37(4):549-556
  9. Chun, H., Y.H. Choi, Y.C. Um, Y. Paek, I.H. Yu, H.Y. You, T.S. Hyun, M.Y. Yon, and Y.S. Shin. 2008. Folate Contents of oriental melon (Cucumis melo) cultivated in greenhouse covered with different films and varieties. J. Bio-Env. Con. 17(1):32-37
  10. Hazra, B., S. Biswas, and N. Mandal. 2008. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern. Med. 8:63 https://doi.org/10.1186/1472-6882-8-63
  11. Hwang, B.H., J.H. Cho, S.S. Ham, and H.Y. Kang. 2000. Chemical analysis of pinus leaves. J. Korean. Soc. Food Sci. Nutr. 29(1):6-9
  12. Hwang, T.Y., Y.J. Park, and K.D. Moon. 2005. Effects of ozone-water washing on the quality of melon. Kor. J. Food Preserv. 12(3):252-256
  13. Jansen, M.C., M. Klerk, F.J. Kok, and P.V. Veer. 2000. Fruits and vegetables in the prevention of cancer and cardiovascular disease. Public Health Nutr. 3(1):103-107 https://doi.org/10.1017/S1368980000000136
  14. Jeffrey, A.M. and G.M. Williams. 2000. Oxidative DNA damage: Endogenous and chemically induced. Regul. Toxicol. Pharmacol. 32(3):283-292 https://doi.org/10.1006/rtph.2000.1433
  15. Kang, H.M., I.L. Choi, and I.S. Kim. 2008. Effect of cultural regions or methods on postharvest physiological characteristics and qualities of qaprika fruits. J. Bio-Env. Con. 17(4):325-329
  16. Kim, B.S., M.J. Kim, and J.H. Choi. 2003. Effects of precooling treatments on the quality of peaches (Mibaek). Kor. J. Food Sci. Technol. 35(6):1233-1236
  17. LI, H.B. and C. Feng. 2001. Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatogra phy with diode array detection. J. Sep. Sci. 24(4):271-274 https://doi.org/10.1002/1615-9314(20010401)24:4<271::AID-JSSC271>3.0.CO;2-L
  18. Maria, A. Anagnostopoulou, P. Kefalas, P. Vassilios, Papageorgiou, N. Andreana, Assimopoulou, and D. Boskou. 2006. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem. 94(1):19-25 https://doi.org/10.1016/j.foodchem.2004.09.047
  19. National Statistical Office. 2008. Annual report on the cause of death statistics. Republic of Korea
  20. Okawa, M., J. Kinjo, T. Nohara, and M. Ono. 2001. DPPH radical scavenging activity of flavonoids obtained from some medicinal plants. Biol. Pharm. Bull. 24(10): 1202-1205 https://doi.org/10.1248/bpb.24.1202
  21. Padayatty, S.J., A. Katz, Y. Wang, P. Eck, O. Kwon, J.H. Lee, S. Chen, C. Corpe, A. Dutta, S.K. Dutta, and M. Levine. 2003. Vitamin C as an antioxidant: Evaluation of its role in disease prevention. J. Am. College Nutr. 22(1):18-35
  22. Park, S.D., Y.S. Shin, S.G. Bae, Y.J. Seo, I.K. Yeon, and H.W. Do. 2004. Colletion of cultivation of oriental melon. Jae-Tak Youn. Seong Ju Fruit vegetable Experiment Station. Korea, pp. 7-13
  23. Park, Y.K., S.H. Choi, S.H. Kim, J.G. Han, and H.G. Chung. 2007. Changes in antioxidant activity, total phenolics and vitamin C content during fruit ripening in Rubus occidentalis. Kor. J. Plant Res. 20(5):461-465
  24. Re, R., N. Pellegrini, A. proteggente, A. Pannala, M. Yang, and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  25. She, G.M., Y.J. Zhang, and C.R. Yang. 2009. Phenolic constituents from Balanophora laxiflora with DPPH radical-scavenging activity. Chem. Biodivers. 6(6):875-880 https://doi.org/10.1002/cbdv.200800139
  26. Shin, Y.S., J.E. Lee, I.K. Yeon, H.W. Do, J.D. Cheung, C.K. Kang, S.Y. Choi, S.J. Youn, J.G. Cho, and D.J. Kwoen. 2008. Antioxidant and antimicrobial effects of extract with water and ethanol of oriental melon (Cucumis melo L. var makuwa Makino). J. Kor. Soc. Appl. Biol. Chem. 51(3):194-199
  27. Youn, A.R., K.H. Kwon, B.S. Kim, S.H. Kim, B.S. Noh, and H.S. Cha. 2009. Changes in quality of muskmelon (Cucumis melo L.) during storage at different temperatures. Kor. J. Food Sci. Technol. 41(3):251-257
  28. Zhishen, J., M. Tang, and J. Wu. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64(4):555-559 https://doi.org/10.1016/S0308-8146(98)00102-2