Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-cancer Activity of Korean Local Plant Extracts Inducing Apoptosis in Various Carcinoma Cells

암세포 특이적 세포 사멸을 유도하는 자생식물 추출물의 항암 효과

  • Yoon, Yi-Kwan (The College of Bio-Resources Science, Dankook University) ;
  • Lee, Seung-Eun (Department of Herbal Crop Research, RDA) ;
  • Lee, Dong-Jin (The College of Bio-Resources Science, Dankook University) ;
  • Rho, Mun-Chual (Bioindustry Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sung, Jung-Suk (Department of National Agrobiodiversity Center, RDA) ;
  • Park, Chung-Berm (Department of Herbal Crop Research, RDA) ;
  • Jang, Young-Joo (Cell cycle & Signal Transduction Laboratory, Department of Nanobiomedical Science & Institute of Tissue Regeneration Engineering, Dankook University)
  • 윤이관 (단국대학교, 생명자원과학대학, 식량생명공학과) ;
  • 이승은 (농촌진흥청 인삼특작부) ;
  • 이동진 (단국대학교, 생명자원과학대학, 식량생명공학과) ;
  • 노문철 (한국생명공학연구원, 생물산업기술연구센터) ;
  • 성정숙 (농촌진흥청 농업유전자원센터) ;
  • 박충범 (농촌진흥청 인삼특작부) ;
  • 장영주 (단국대학교, 나노바이오의과학과, 세포주기신호전달연구실 & 조직재생공학연구소)
  • Published : 2009.03.31
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Abstract

Thirty five methanol extracts from 19 natural local plants, which have been used as traditional anti-cancer medicine, were prepared. They were analyzed the cytotoxic effects on primary fibroblast cells and carcinoma cells. The root extract of Solanum nigrum were highly toxic in both cell lines with $IC_{50}$ values of less than $0.01{\mu}g/{\mu}l$, and 26 of 35 extracts were toxic in all cells with $IC_{50}$ values of $0.1{\sim}2{\mu}g/{\mu}l$. Three extracts including the fruit extracts of Solanum nigrum and Morus alba had no cytotoxic activity in both cell lines. Five of 35 extracts were highly toxic in cancer cells than in primary cells. Because primary cells were more resistant on these extracts, the five extracts were selected for anti-cancer agent candidates. Apoptosis or programmed cell death has an essential role in chemotherapy-induced tumor cell killing. Recently, inducers of apoptosis have been used in cancer therapy. When two of 5 cancer cell-specific cytotoxic extracts (Ulmus parvifolia and Zelkova serrata) were treated in concentration of $0.02{\sim}0.1{\mu}g/{\mu}l$, apoptosis were increased at 3-5 times in cancer cell lines. Finally, the apoptotic effects of these extracts were confirmed by cleavages of both poly-(ADP-ribose)-polymerase and caspase-3 as apoptotic markers. In this report, we suggested that two of 35 medicinal herb extracts can be useful anti-cancer drug candidates inducing apoptosis in several carcinoma cell lines.

Keywords

References

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